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Ultrafast, on-chip PCR could speed diagnosis during current and future pandemics

Date:
May 26, 2021
Source:
American Chemical Society
Summary:
Researchers have developed a plasmofluidic chip that can perform PCR in only about 8 minutes, which could speed diagnosis during current and future pandemics.
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Reverse transcription-polymerase chain reaction (RT-PCR) has been the gold standard for diagnosis during the COVID-19 pandemic. However, the PCR portion of the test requires bulky, expensive machines and takes about an hour to complete, making it difficult to quickly diagnose someone at a testing site. Now, researchers reporting in ACS Nano have developed a plasmofluidic chip that can perform PCR in only about 8 minutes, which could speed diagnosis during current and future pandemics.

Rapid diagnosis of COVID-19 and other highly contagious viral diseases is important for timely medical care, quarantining and contact tracing. Currently, RT-PCR -- which uses enzymes to reverse transcribe tiny amounts of viral RNA to DNA, and then amplify the DNA so that it can be detected by a fluorescent probe -- is the most sensitive and reliable diagnostic method. But because the PCR portion of the test requires 30-40 cycles of heating and cooling in special machines, it takes about an hour to perform, and samples must typically be sent away to a lab, meaning that a patient usually has to wait a day or two to receive their diagnosis. Ki-Hun Jeong and colleagues wanted to develop a plasmofluidic PCR chip that could quickly heat and cool miniscule volumes of liquids, allowing accurate point-of-care diagnosis in a fraction of the time.

The researchers devised a postage stamp-sized polydimethylsiloxane chip with a microchamber array for the PCR reactions. When a drop of sample is added to the chip, a vacuum pulls the liquid into the microchambers, which are positioned above glass nanopillars with gold nanoislands. Any microbubbles, which could interfere with the PCR reaction, diffuse out through an air-permeable wall. When a white LED is turned on beneath the chip, the gold nanoislands on the nanopillars quickly convert light to heat, and then rapidly cool when the light is switched off. The researchers tested the device on a piece of DNA containing a SARS-CoV-2 gene, accomplishing 40 heating and cooling cycles and fluorescence detection in only 5 minutes, with an additional 3 minutes for sample loading. The amplification efficiency was 91%, whereas a comparable conventional PCR process has an efficiency of 98%. With the reverse transcriptase step added prior to sample loading, the entire testing time with the new method could take 10-13 minutes, as opposed to about an hour for typical RT-PCR testing. The new device could provide many opportunities for rapid point-of-care diagnostics during a pandemic, the researchers say.

The authors acknowledge funding from the Korea Advanced Institute of Science and Technology (KAIST) and the National Research Foundation of Korea.


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Materials provided by American Chemical Society. Note: Content may be edited for style and length.


Journal Reference:

  1. Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong. Ultrafast and Real-Time Nanoplasmonic On-Chip Polymerase Chain Reaction for Rapid and Quantitative Molecular Diagnostics. ACS Nano, 2021; DOI: 10.1021/acsnano.1c02154

Cite This Page:

American Chemical Society. "Ultrafast, on-chip PCR could speed diagnosis during current and future pandemics." ScienceDaily. ScienceDaily, 26 May 2021. <www.sciencedaily.com/releases/2021/05/210526132118.htm>.
American Chemical Society. (2021, May 26). Ultrafast, on-chip PCR could speed diagnosis during current and future pandemics. ScienceDaily. Retrieved November 22, 2024 from www.sciencedaily.com/releases/2021/05/210526132118.htm
American Chemical Society. "Ultrafast, on-chip PCR could speed diagnosis during current and future pandemics." ScienceDaily. www.sciencedaily.com/releases/2021/05/210526132118.htm (accessed November 22, 2024).

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