New artificial enzyme breaks down tough, woody lignin

Reporting in the journal Nature Communications, a team of researchers from Washington State University and the Department of Energy's Pacific Northwest National Laboratory showed that their artificial enzyme succeeded in digesting lignin, which has stubbornly resisted previous attempts to develop it into an economically useful energy source.

Lignin, which is the second most abundant renewable carbon source on Earth, mostly goes to waste as a fuel source. When wood is burned for cooking, lignin byproducts help impart that smoky flavor to foods. But burning releases all that carbon to the atmosphere instead of capturing it for other uses.

"Our bio-mimicking enzyme showed promise in degrading real lignin, which is considered to be a breakthrough," said Xiao Zhang, a corresponding author on the paper and associate professor in WSU's Gene and Linda Voiland School of Chemical Engineering and Bioengineering. Zhang also holds a joint appointment at PNNL. "We think there is an opportunity to develop a new class of catalysts and to really address the limitations of biological and chemical catalysts."

Lignin is in all vascular plants, where it forms cell walls and provides plants with rigidity. Lignin allows trees to stand, gives vegetables their firmness and makes up about 20-35% of the weight of wood. Because lignin turns yellows when exposed to air, the wood products industry removes it as part of the fine papermaking process. Once removed, it is often inefficiently burned to produce fuel and electricity.

Chemists have tried and failed for more than a century to make valuable products from lignin. That track record of frustration may be about to change.

One better than nature

"This is the first nature-mimetic enzyme which we know can efficiently digest lignin to produce compounds that can be used as biofuels and for chemical production," added Chun-Long Chen, a corresponding author, a Pacific Northwest National Laboratory researcher, and affiliate professor in chemical engineering and chemistry at the University of Washington.

In nature, fungi and bacteria are able to break down lignin with their enzymes, which is how a mushroom-covered log decomposes in the forest. Enzymes offer a much more environmentally benign process than chemical degradation, which requires high heat and consumes more energy than it produces.

But, natural enzymes degrade over time, which makes them hard to use in an industrial process. They're expensive, too.

"It's really hard to produce these enzymes from microorganisms in a meaningful quantity for practical use," said Zhang. "Then once you isolate them, they're very fragile and unstable. But these enzymes offer a great opportunity to inspire models that copy their basic design."

While researchers have been unable to harness natural enzymes to work for them, they have over the decades learned a lot about how they work. A recent review article by Zhang's research team outlines the challenges and barriers toward the application of lignin degrading enzymes. "Understanding these barriers provides new insights toward designing biomimetic enzymes," Zhang added.

Peptoid scaffold is key

In the current study, the researchers replaced the peptides that surround the active site of natural enzymes with protein-like molecules called peptoids. These peptoids then self-assembled into nanoscale crystalline tubes and sheets. Peptoids were first developed in the 1990s to mimic the function of proteins. They have several unique features, including high stability, that allow scientists to address the deficiencies of the natural enzymes. In this case, they offer a high density of active sites, which is impossible to obtain with a natural enzyme.

"We can precisely organize these active sites and tune their local environments for catalytic activity," said Chen, "and we have a much higher density of active sites, instead of one active site."

As expected, these artificial enzymes are also much more stable and robust than the natural versions, so that they can work at temperatures up to 60 degrees Celsius, a temperature that would destroy a natural enzyme.

"This work really opens up new opportunities," said Chen. "This is a significant step forward in being able to convert lignin into valuable products using an environmentally benign approach."

If the new bio-mimetic enzyme can be further improved to increase conversion yield, to generate more selective products, it has potential for scale up to industrial scale. The technology offers new routes to renewable materials for aviation biofuel and biobased materials, among other applications.

The research collaboration was facilitated through the WSU-PNNL Bioproducts Institute. Tengyue Jian, Wenchao Yang, Peng Mu, Xin Zhang of PNNL and Yicheng Zhou and Peipei Wang of WSU also contributed to the research.

The work was funded by the state of Washington's Joint Center for Aerospace Technology and Innovation, a program that supports industry and university research collaborations to develop innovative technologies in the aerospace industry, and by the Department of Energy, Office of Science, Office of Basic Energy Sciences as part of the Center for the Science of Synthesis Across Scales, an Energy Frontier Research Center located at the University of Washington. Additional support was provided by the National Science Foundation (1454575) and the Department of Agriculture National Institute of Food and Agriculture (2018-67009-27902). Peptoid synthesis capabilities were supported by the Materials Synthesis and Simulation Across Scales Initiative, a Laboratory Directed Research and Development program at PNNL.