Researchers identify an immunotherapy target to combat glioblastomas

Their in-depth molecular analysis of over 200,000 single cells revealed a protein, called S100A4, that could be a potential therapeutic target for restoring antitumor action of immune cells toward glioblastomas that have otherwise tricked the immune system into protecting it.

The study, titled "Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target," was recently published in Nature Communications, and advances the search for targeted therapies for heterogeneous tumors, which contain different types of tumor and normal cells mixed within a single mass. These heterogeneous cancers are notoriously difficult to manage, because treatments that work against one group of tumor cells may be completely ineffective in others.

Around 48% of all primary malignant brain tumors are glioblastomas, and more than 10,000 people in the United States will succumb to the disease each year. The highly invasive brain cancer cells infiltrate the brain extensively, making surgical resection a very big challenge. Adding to the complexity of the disease is this cancer's ability to rapidly mutate, so even in different locations in the brain of the same patient, glioblastoma encompasses a mosaic of cancer cell types, posing a major setback for targeted therapies.

As in the case with most diseases affecting the brain, the blood-brain barrier poses another challenge for drug delivery. In glioblastoma, the blood-brain barrier is weakened, allowing immune cells from the periphery to permeate the central nervous system. Mysteriously, however, glioblastomas tend to selectively attract or turn most immune cells that infiltrate the tumor to immune suppressive cells and promote their malignancy.

"A lot of immunotherapies currently target the reactivation of effector T cells, which are important to attack and eliminate cancer cells, but in glioblastoma, the effector T cell infiltration is, in fact, very low," said Kyuson Yun, Ph.D., senior author on the study with the Houston Methodist Research Institute and associate professor of neurology at the Houston Methodist Academic Institute. "Instead, there is an overabundance of immunosuppressive myeloid cells in glioblastomas."

To investigate the complex immune-cancer cell interactions, the researchers conducted comprehensive genetic profiling of different cell types in 44 samples of glioblastoma from 18 patients. For each patient, they analyzed glioblastoma tissue from different parts of the brain tumor to gain insights into the cancer's heterogeneity within each patient. Then, they performed high throughput single-cell RNA sequencing to catalog individual cells based on their gene expression of different molecules.

Upon grouping the cells based on their molecular profiles, the researchers found that glioblastoma cells within and across patients could be categorized into nine groups based on their cellular state independent of the specific mutations in individual cells. They further identified nine subtypes of myeloid cells in glioblastoma, including the brain's primary immune cells, the microglia, that are associated with better patient outcomes. The tumors were also filled with bone marrow-derived macrophages and regulatory T cells (Tregs) that are immunosuppressive and are linked with worse patient outcomes.

Hence, the researchers turned their attention to identifying a molecule activated in immune suppressive Tregs and myeloid cells. Their strategy was to spare "good" immune cells that are associated with better survival and selectively target "bad" immune cells that promote tumor growth and immune evasion. They discovered that the S100A4 regulator protein is produced and secreted by glioblastoma cancer cells, immunosuppressive T cells and bone marrow-derived myeloid cells.

Yun said her team plans to develop antibody drugs to target this S100A4 protein so that the glioblastoma's tight grip on regulatory T cells and bone marrow-derived macrophages can be loosened. In addition, they plan to develop small molecules that can enter the nucleus of cancer cells and inhibit the function of the S100A4 protein in glioblastoma stem cells.

"Right now, therapies take a sledgehammer approach since there has been a lack of understanding about which myeloid cell types promote glioblastoma growth and which ones inhibit it," Yun said. "Single-cell sequencing allowed us to define the heterogeneous myeloid cell types and identify the molecular characteristics of immune cells that promote or suppress tumor growth and consequently allowed us to selectively manipulate immune suppressive cells to restore the tumor-flighting action of the immune system."

She added that in the next few years single-cell datasets such as those in this study will dramatically change the understanding of human cancer and guide efforts toward the development of new generations of anti-cancer drugs, particularly for immunotherapies.

Yun's collaborators on this study were Nourhan Abdelfattah, Parveen Kumar, Caiyi Wang, Jia-Shiun Leu, William F. Flynn, Ruli Gao, David S. Baskin, Kumar Pichumani, Omkar B. Ijare, Stephanie L. Wood, Suzanne Z. Powell, David L. Haviland, Brittany C. Parker Kerrigan, Frederick F. Lang, Sujit S. Prabhu, Kristin M. Huntoon, Wen Jiang, Betty Y. S. Kim and Joshy George.

This study was supported by grants from the National Institutes of Health (1R01NS121405), Cancer Prevention & Research Institute of Texas (RP180882), Department of Defense (W81XWH-14-1-0115), the Houston Methodist Foundation, the Donaldson Charitable Foundation, The Peak Foundation, the Department of Defense Horizon Award (CA191052), The University of Texas MD Anderson Moon Shots Program^TM, the National Cancer Institute (P50CA127001), The Broach Foundation for Brain Cancer Research and The Elias Family Fund.