Novel nanoparticle-based approach detects and treats oral plaque without drugs

A team of researchers from the University of Illinois has recently devised a practical nanotechnology-based method for detecting and treating the harmful bacteria that cause plaque and lead to tooth decay and other detrimental conditions.

Bioengineering Associate Professor Dipanjan Pan (seated) and doctoral student Fatemeh Ostadhossein have demonstrated a drug-free, nanotechnology-based method for detecting and destroying the bacteria that causes dental plaque.

Oral plaque is invisible to the eye so dentists currently visualize it with disclosing agents, which they administer to patients in the form of a dissolvable tablet or brush-on swab. While useful in helping patients see the extent of their plaque, these methods are unable to identify the difference between good and bad bacteria.

"Presently in the clinic, detection of dental plaque is highly subjective and only depends on the dentist's visual evaluation," said Bioengineering Associate Professor Dipanjan Pan, head of the research team. "We have demonstrated for the first time that early detection of dental plaque in the clinic is possible using the regular intraoral X-ray machine which can seek out harmful bacteria populations."

In order to accomplish this, Fatemeh Ostadhossein, a Bioengineering graduate student in Pan's group, developed a plaque detection probe that works in conjunction with common X-ray technology and which is capable of finding specific harmful bacteria known as Streptococcus mutans (S. mutans) in a complex biofilm network. Additionally, they also demonstrated that by tweaking the chemical composition of the probe, it can be used to target and destroy the S. mutans bacteria.

The probe is made up of nanoparticles made of hafnium oxide (HfO₂), a non-toxic metal that is currently under clinical trial for internal use in humans. In their study, the team demonstrated the efficacy of the probe to identify biochemical markers present at the surface of the bacterial biofilm and simultaneously destroy S. mutans. They conducted their study on Sprague Dawley rats.

In practice, Pan envisions a dentist applying the probe on the patient's teeth and using the X-ray machine to accurately visualize the extent of the biofilm plaque. If the plaque is deemed severe, then the dentist would follow up with the administering of the therapeutic HfO₂ nanoparticles in the form of a dental paste.

In their study, the team compared the therapeutic ability of their nanoparticles with Chlorhexidine, a chemical currently used by dentists to eradicate biofilm. "Our HfO₂ nanoparticles are far more efficient at killing the bacteria and reducing the biofilm burden both in cell cultures of bacteria and in [infected] rats," said Ostadhossein, noting that their new technology is also much safer than conventional treatment.

The nanoparticles' therapeutic effect is due, said Pan, to their unique surface chemistry, which provides a latch and kill mechanism. "This mechanism sets our work apart from previously pursued nanoparticle-based approaches where the medicinal effect comes from anti-biotics encapsulated in the particles," said Pan, also a faculty member of the Carle Illinois College of Medicine and the Beckman Institute for Advanced Science and Technology. "This is good because our approach avoids anti-biotic resistance issues and it's safe and highly scalable, making it well-suited for eventual clinical translation."

In addition to Pan and Ostadhossein, other members of the research team include bioengineering post-doctoral researcher Santosh Misra, visiting scholar Indu Tripathi, undergraduate Valeriya Kravchuk, visiting scholar Gururaja Vulugundam; and Veterinary Medicine clinical assistant professor Denae LoBato and adjunct assistant professor Laura Selmic.

Their work is described in the paper, "Dual purpose hafnium oxide nanoparticles offer imaging Streptococcus mutans dental biofilm and fight it In vivo via a drug free approach," published online on July 30, 2018, in the journal Biomaterials. The research was funded by the University of Illinois at Urbana-Champaign Children's Discovery Institute and the American Heart Association.