'Ecologically diverse' breast cancers more likely to be deadly

The test could be used in the clinic to assess how likely women's breast cancers are to be aggressive, and to help tailor treatment accordingly.

Scientists at The Institute of Cancer Research, London, developed a test called the Ecosystem Diversity Index which combines methods used by ecologists with a powerful cancer imaging technique to pick out cancerous cells from normal cells in tumours.

The test identified several particularly aggressive subgroups of breast cancer and was a stronger predictor of survival than many established markers for the disease. It was effective at predicting survival chances in high-grade tumours.

The study is published in the journal PLoS Medicine, and was funded by The Institute of Cancer Research (ICR) itself, Breast Cancer Now, the Wellcome Trust and Cancer Research UK.

Researchers combined software taken from the study of ecology with image analysis and examined 1,026 samples of untreated breast tumours from three different hospitals.

They analysed three distinct cell types -- cancer cells, immune system lymphocytes and stromal cells, which produce connective tissue -- in place of species of animals and plants in natural ecosystems.

Patients with high-grade tumours larger than five centimetres in diameter whose tumours scored high on the Ecosystem Diversity Index had a 16 per cent chance of surviving five years, compared with 50 per cent for tumours with low diversity scores.

The ICR researchers also looked at the Ecosystem Diversity Index in addition to genetic factors -- the first time a study has analysed the impact of different cell types around a tumour together with genetic information.

They found that patients who had tumours with a high diversity score together with mutations in the p53 tumour suppressor gene had especially poor survival chances.

Study leader Dr Yinyin Yuan, Team Leader in the Centre for Evolution and Cancer at The Institute of Cancer Research, London, said:

"Our findings show that mathematical models of ecological diversity can spot more aggressive cancers. By analysing images of the environment around a tumour based on Darwinian natural selection principles, we can predict survival in some breast cancer types even more effectively than many of the measures used now in the clinic.

"In the future, we hope that by combining cell diversity scores with other factors that influence cancer survival, such as genetics and tumour size, we will be able to tell apart patients with more or less aggressive disease so we can identify those who might need different types of treatment."

Dr Rachael Natrajan, Team leader in the Breast Cancer Now Toby Robins Research Centre at The Institute of Cancer Research, London, said:

"We have known for a while that genetic diversity between cancer cells in tumours is associated with more aggressive disease, and our new results also show that diversity of cells within the tumour microenvironment also contributes to aggressive breast cancer. Integration of our new measure with genetic alterations can actually provide additional prognostic information from just genetics alone, and may pave the way for a new wave of diagnostic biomarkers."

Professor Paul Workman, Chief Executive of The Institute of Cancer Research, London, said:

"This ingenious study has combined techniques developed in ecology and cell biology with computer vision technology to develop a totally new predictive test which could ultimately be used for patients in the clinic.

"It teaches us a valuable lesson -- we should always remember that cancer cells are not developing and growing in isolation, but are part of a complex ecosystem that involves normal human cells too. By better understanding these ecosystems, we aim to create new ways to diagnose, monitor and treat cancer."