Gene analysis generates spatial map of intestinal cells and traces their trajectories during gut inflammation

They identified cell populations associated with healthy and inflamed states, mapped their spatial neighborhoods, and traced the evolution of these populations during the inflammation process. One significant cell type to note was fibroblasts, that, when in a distressed state, can induce inflammation associated fibroblasts (IAFs), which may help remodel extracellular matrixes, recruit immune cells, and produce inflammatory cytokines.

However, some key questions still remain, including the specific diversity of IAF populations, their precise tissue locations, and how they emerge during inflammation. Nevertheless, the researchers were able to generate a unique spatial atlas of a mouse colon both in a healthy state, and during intestinal inflammation, which has the potential to assist with therapeutic treatments for chronic inflammatory diseases. In previous studies, IAFs have been observed in a variety of diseases, including cancers and autoimmune diseases, so understanding the role and mechanisms of IAFs may help with the development of treatments for these other diseases.

"Our team wanted to better understand how cells are organized within the gut, and how inflammation can impact cellular interactions and communication at the tissue scale," said senior author Roni Nowarski, PhD, of the Brigham's Department of Neurology and Harvard Medical School's Department of Immunology. "This work is particularly exciting and has given us a better understanding of the tissue context of cellular responses during inflammation, which we hope will help us design better therapeutics to fight serious chronic inflammatory diseases."