Scientists use zebrafish to understand the connection between the immune system and regeneration

New research from the Stowers Institute for Medical Research used the highly regenerative zebrafish to investigate the timing and genetic programs of macrophages, a type of white blood cell, in the repair and regeneration of a zebrafish sensory organ. Understanding how the immune system responds to injury, first by inducing inflammation immediately followed by an anti-inflammatory response, provides invaluable knowledge for designing targeted immunotherapies that may be applicable in combating human conditions like hearing loss or deafness, heart or spinal cord damage.

Recently published in Nature Communications on September 20, 2022, Postdoctoral Researcher Nicolas Denans, PhD, in the lab of Stowers Investigator Tatjana Piotrowski, PhD, discovered a new macrophage anti-inflammatory paradigm. Rather than the established view that anti-inflammatory activation states for macrophages are linked to just one type of signaling pathway, Denans found that the same population can, and must, transition through each of three anti-inflammatory states for organ regeneration.

"Organ regeneration offers an exciting opportunity for studying the immune system and to inquire why some species can regenerate organs like the heart or missing limbs while others like humans cannot," said Piotrowski.

Zebrafish sensory organ hair cells are an ideal system to investigate the pathways and cell types involved in regeneration since they are easily destroyed with antibiotics and begin regenerating within five hours. This enabled the researchers to identify the exact timing and genetic programs for each anti-inflammatory macrophage activation state.

"Our hypothesis is that human macrophages do not receive the proper chemical activation "cocktail" to instruct pro-regenerative processes," said Denans. "Identifying the molecular recipe of macrophage activation in zebrafish may one day enable us to design regenerative immunotherapies in humans."

Essential for organ regeneration, macrophages, which in Latin literally translates as "big eaters," engulf foreign particles like dead cells and bacteria and use enzymes to digest them. In addition to their culinary appetite, these cells signal both pro- and anti-inflammatory pathways to secrete chemicals, or cytokines to either recruit additional types of white blood cells or trigger anti-inflammatory pathways for cellular and tissue repair.

Investigating macrophages at high spatial resolution and at multiple closely spaced time points during zebrafish sensory hair cell death and regeneration was critical. For the first time, the study demonstrates that a single population of this cell type sequentially and independently transitions through three different anti-inflammatory states, each with its own unique molecular and genetic signature.

"The new evidence is a valuable resource for comparative studies on the genetic programs involved in macrophage-mediated repair and regeneration," said Denans. "In other words, different types of injuries may induce different kinds of inflammatory responses. We want to decipher whether this "language" is universal or if there are a variety of dialects."

While the study marks the first time that the sequential macrophage states have been resolved with extraordinary precision, preliminary comparisons with previously reported pathways in different organs and species suggest that this mechanism is likely conserved.

"When you look in more detail, macrophages are not only required to initiate regeneration, but they also interact with the brain by communicating with nerve cells to reestablish and maintain synapses necessary for proper organ function after regeneration," said Piotrowski.

The team hopes that additional studies based on this new finding may provide the basis for designing tailored immunotherapies to diminish disease, perhaps enabling greater regenerative abilities in regeneration-limited animals like humans.

"This is just one in a series of steps to entertain the idea of developing regenerative immunotherapies in humans," said Denans.

Additional authors include Nhung T. T. Tran, Madeleine Swall, Daniel C. Diaz, and Jillian Blanck.

This work was funded by the National Institute on Deafness and Other Communication Disorders of the National Institutes of Health (award 1R01DC015488-01A1) and by institutional support from the Stowers Institute for Medical Research. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.