Separating tiny bacteria by shape: Simple tech for E. coli sorting

The task of separating tiny bacteria based on their shape is a vital step in scientific and clinical applications, but also a difficult one. Whether an E. Coli bacterium is in a round or elongated configuration can indicate its state of biological function, and the ability to prepare homogeneous populations with uniform shapes may help improve experimental reproducibility. Moreover, being able to sort samples into sub-populations based on their shape may help diagnose patient health or assess environmental contamination. However, this ability has been difficult to achieve, especially at the scales needed to be practical.

Now, a team of researchers at NAIST and has been able to separate drug-treated E. coli bacteria using microfluidic channel containing poly-ethylene-oxide (PEO), a viscoelastic fluid. The elongation of the bacteria, as measured by the aspect ratio, ranged from 1.0 (spherical) to 5.5 (rod-shaped). Microchannels made of poly(dimethylsiloxane) were created with photolithography. PEO, which has both high viscosity and elasticity, was made to flow along the edges of an expanding V-shaped chamber. The elongated bacterium followed this flow to be collected at the edges of the device. "The shape of a E. coli bacterium changes based on its attachment, motility, dispersal, predation and differentiation behaviors," author Yalikun Yaxiaer explains. A rod-like configuration is more efficient for swimming, while a spherical shape may help it form communities. These changes are important milestones for the progression of disease, and whether the bacteria are swimming free or forming communities. "This technology will allow downstream genomics experiments on the separated E. coli populations to determine linkage between cell shape, antibiotic susceptibility and gene function," author Yoichiroh Hosokawa says. The team hopes the microfluidic platform they developed will be adopted by the broader scientific community for different applications in the fields of microbiology, molecular biology and biomedicine.