Scientists explore tick salivary glands as tool to study virus transmission, infection

Flaviviruses include dengue virus, Zika virus, West Nile virus, yellow fever virus, Powassan virus and several other viruses. Powassan is the only endemic flavivirus spread by ticks in North America, where it is considered a re-emerging virus. Physicians in the United States have reported roughly 100 cases of disease in the past decade, half of them in 2016-17. Powassan virus disease occurs primarily in northeastern states and the Great Lakes region. Though disease caused by Powassan virus is rare -- most people who become infected with Powassan virus do not develop any symptoms -- the virus can be transmitted very rapidly. Within 15 minutes, an infected tick can transmit the virus to a person or other mammal on which it is feeding. Symptoms of Powassan virus disease can include fever, headache, vomiting, weakness, confusion, loss of coordination, speech difficulties, and seizures. If the virus infects the central nervous system, it can cause brain inflammation and meningitis. Debilitating long-term neurological problems or even death may occur.

In examining the molecular interactions between black-legged ticks and mammals, the NIAID scientists have learned that flaviviruses reproduce in specific locations in tick salivary gland cultures. This could explain why virus transmission occurs so quickly. They also noted that only certain types of salivary gland cells are infected, and they identified a specific tick gene that is involved in infection. Taken together, these findings help identify transmission pathways that potentially could be blocked with a countermeasure. The group also is assessing how viruses grow in cells of the cultured tick midgut to help identify different viruses that can grow in black-legged ticks.