Green tea compound helps siRNA slip inside cells

Small interfering RNAs (siRNAs) have great therapeutic potential because they can dial down the expression of disease-related genes. However, getting siRNAs into cells where they can do their job has been challenging. Being relatively large and negatively charged, siRNAs cannot easily cross the cell membrane, and they are susceptible to degradation by RNA-chomping enzymes. To overcome these problems, some researchers have tried coating siRNAs with various polymers. However, most small polymers can't shuttle siRNAs into cells, whereas larger polymers can be effective but are generally toxic. Yiyun Cheng and colleagues wondered if they could use EGCG, which is known to bind strongly to RNA, in combination with a small polymer to form nanoparticles that safely deliver siRNA into cells.

The team made their nanoparticles by first combining EGCG and siRNA, which self-assembled into a negatively charged core. Then, the researchers coated this core with a shell consisting of a small, positively charged polymer. These nanoparticles efficiently knocked down the expression of several target genes in cultured cells, showing that the particles could cross the cell membrane. Next, the researchers tested the nanoparticles in a mouse model of intestinal injury, using an siRNA that targeted a pro-inflammatory enzyme. The nanoparticles improved symptoms such as weight loss, shortening of the colon and intestinal inflammation. In addition to the gene-silencing effects of the siRNA, EGCG could contribute to the nanoparticles' effectiveness through its antioxidant and anti-inflammatory properties, the researchers say.