Helping in spite of risk: Ants perform risk-averse sanitary care of infectious nest mates

In their colonies, ants such as the invasive garden ant Lasius neglectus live in a very confined space. This carries the risk that diseases can spread rapidly and threaten the entire colony. Sylvia Cremer and her group have already shown in previous studies that ants of the species Lasius neglectus fend off the pathogenic fungus Metarhizium by intensively cleaning and caring for colony members whose bodies have been contaminated by fungal spores. In the current study, Sylvia Cremer and her group addressed the question of how the caring ants protect themselves from infection.

Care means protection and risk

There are two ways for ants to care for nest mates: either by grooming off pathogens or by chemically disinfecting them. However, the extensive contact between contaminated and caring ants during care may lead to a transmission of the pathogen, which often induces low-level infections in the caring individual that do not cause disease. As the research team has shown in a previous study, such low-level infections of the caring ants stimulate their immune system and can lead to a protective effect against future infection, similar to the early form of vaccination used by humans, termed variolation. If this ant comes in contact with the same pathogen again in the future, its defense against the fungal pathogen is already upregulated, and the course of the disease is mild. However, in the current study the research team showed that this immunization caused by low-level infections, unlike modern vaccinations in humans, has a cost. If the ant comes in contact with a second, different pathogen, it is not only unprotected, but is even more susceptible to the second pathogen, which can subsequently cause a highly detrimental, superinfection.

Flexible care protects against superinfection

Although ants with low-level infections are more susceptible to superinfections, the researchers show that this altered disease susceptibility affects how ants care for their infectious nest mates. They continue to perform care, but alter how they do so to decrease their risk of contracting a second infection. This risk avoidance is flexible and depends on the current immune status of the ant. If an ant is protected against a pathogen because it is currently immunized, it grooms the infected nestmate more than non-immunized ants. "This close contact means that the caring ant is exposed to a large number of fungal spores from the infectious nest mates, but it is less susceptible to them because of previous immune stimulation," explains Sylvia Cremer.

The situation is different when the ant encounters a nest mate carrying a pathogen that the caring ant is susceptible to. If the ant has developed a susceptibility to pathogen B due to a previous infection with pathogen A, then it sprays the contaminated nestmate carrying pathogen B with formic acid to neutralize the pathogen. This avoids the need for grooming and the close contact that comes with it, preventing pathogen transmission and protecting the caring ant from superinfection. "This risk-averse care improves and maintains the health of the caring animals and thus of the whole colony. In humans, nursing staff and doctors also pay attention to their immune status, for example by vaccinating before entering a dangerous zone. Importantly, ants are capable of this adjustment without the need for vaccination records that humans typically rely on" explains Sylvia Cremer.

Matthias Konrad and Christopher Pull are the first authors of the study. Matthias Konrad was a PhD student supervised by Sylvia Cremer from 2009 to 2014, and joined IST Austria in 2010 as one of the first PhD students, and stayed for one year after his PhD as a postdoc in the Cremer group. Christopher Pull was a PhD student in the group of Sylvia Cremer at IST Austria from 2012 to 2017, and is now a postdoc at the Royal Holloway University, London. Sylvia Cremer studies the social immune system in ants with the aim of finding out more about epidemiology and disease dynamics in societies.