Bacterial boost for bio-based fuels

"We are solving multiple problems at the same time," said ORNL researcher Abhijeet Borole, who led a multi-year project to develop the system.

The team's lab-scale demonstration can produce 11.7 liters of hydrogen per day at rates that are required for industrial applications. Borole notes that although more work is required to bring the technology to the commercial scale, their progress demonstrates the potential of microbial electrolysis to make bio-refineries more efficient and economically viable.

Much like a conventional petroleum refinery, the bio-refinery concept is focused on the conversion of plant materials into higher value products, including hydrocarbon fuels and chemicals.

Microbial electrolysis is powered by electrogens -- bacteria that digest organic compounds and generate an electric current. Borole put these bacteria to work in breaking down organic acids in liquid bio-oil that is produced from plant feedstocks such as switchgrass. Normally, about a quarter of the liquid bio-oil is contaminated water that contains corrosive acids.

"We are taking this waste, which can be 20 to 30 percent of the biomass that you put into the process, making hydrogen from it and putting that hydrogen back into the oil," Borole said.

The hydrogen generated from the microbes could displace the need for natural gas, which is used later in the production process to upgrade bio-oil into more desirable drop-in liquid fuels.

"You can recycle the water, produce clean hydrogen and eliminate the natural gas," Borole said.

The researchers developed a procedure to evolve and enrich a hardy bacterial community that could tolerate the toxic compounds in the biofuel wastewater. This delicate balance also involved optimizing the overall process and system parameters to enable the bacteria's success.

"You are trying to efficiently extract electrons from hundreds of compounds and make hydrogen," Borole said. "How do you do that when the plant byproducts are poisoning this bacterial food? You have to find a way to negate or neutralize that poison and be able to produce those electrons at the same time."

In this application, the bacterial poison comes in the form of products created by the degradation of lignin, a tough polymer found in plant cell walls. But understanding how to build and optimize microbial electrolysis systems that can tolerate and treat contaminated wastewater could have benefits outside of biofuel production.

"These systems have potential for wide-ranging applications, including energy production, bioremediation, chemical and nanomaterial synthesis, electro-fermentation, energy storage, desalination and produced water treatment," said Alex Lewis, a doctoral student with the University of Tennessee's Bredesen Center for Interdisciplinary Research and Education.

The research team is now focused on completing a life-cycle analysis for the technology to evaluate its greenhouse gas emissions and water use.