Today's most successful fish weren't always evolutionary standouts

Evolutionary biologists and paleontologists since Darwin have offered hypotheses to explain why teleosts seem to have "out-evolved" other groups. The closely related holosteans, for example, once dominated the oceans but are now considered "living fossils," representing just eight species in forms that resemble those of the past.

But this view of the teleost success story may be based on the false premise that teleosts dominate today because they have always been more evolutionarily innovative than other groups. A new analysis of more than a thousand fossil fishes from nearly 500 species led by the University of Pennsylvania's John Clarke revealed that the teleosts' success story is not as straightforward as once believed. Examining the first 160 million years of teleost and holostean evolution, from the Permian to the early Cretaceous periods, the scientists show that holosteans were as evolutionarily innovative as teleosts, and perhaps even more so.

"A lot of these so-called living fossils that appear to be kind of boring today actually have a pretty rich history," said Clarke. "If we were to go back in time to the Triassic and you had to place a bet on which group was going to do better going forward, you would have definitely chosen the holosteans. It just didn't work out that way."

Clarke collaborated with Graeme T. Lloyd of Macquarie University and Matt Friedman of the University of Oxford on the work, which appears in Proceedings of the Academy of Natural Sciences.

It's easy to see why scientists have long presumed teleosts exceptional. They represent 29,000 diverse species worldwide, roughly half of modern vertebrates. In contrast, the eight living species that comprise holosteans share a resemblance, and all dwell in the freshwaters of eastern North America. Numerous ideas have been put forward to explain teleost success, including the flexible structure of their jaws, a diversity of reproductive strategies and the symmetry of their tail fins.

With the emergence of molecular and genetic techniques to probe evolution, researchers have also attributed teleost success to a genome duplication event in the evolutionary past that left the fish with twice the number of chromosomes and thus more raw material with which to acquire beneficial mutations and to evolve.

Yet Clarke and colleagues wanted to back up a bit, questioning the very assumption that teleosts had always been more evolutionarily innovative and successful.

"There were times in the past when holosteans were top dog," Clarke said. "There are lots of holostean fossils, and they were quite diverse, not only in numbers but in the wide variety of sizes and shapes they possessed."

It was known from the fossil record that holosteans appeared to be more dominant in the Triassic Period on into the Early and Middle Jurassic. In the Late Jurassic, however, teleosts began to take over.

The researchers decided, therefore, to focus on the earlier period of fish evolution, starting in the Permian, which just preceded the Triassic period, and following it through 160 million years into the Early Cretaceous, which followed the Jurassic.

To do so, they relied on a dataset that included the size and shape of hundreds of fossils Clarke had compiled during visits to 15 museums as part of his Ph.D. research. They also constructed "supertrees," to summarize the relationships of nearly all known extinct species of holosteans and teleosts from the Triassic, Jurassic and Early Cretaceous. These large trees were built from more than 100 smaller trees already available in the paleontology literature, from studies that examined the morphological traits of fishes to work out their evolutionary tree.

While other researchers have examined patterns of diversity in fish fossils, no one had ever applied a quantitative framework to determine whether holostean or teleost fishes possessed higher rates, or greater innovation, in shape and size.

The Penn-led scientists were able to use the supertrees to evaluate first the rate of size evolution in teleosts versus holosteans and then to compare the degree of shape innovation in the two groups.

In their various analyses of the specimens, Clarke and colleagues found no support for the expectation that teleosts would change their body sizes and shapes faster, or be better able to "invent" new sizes and shapes compared with holosteans. On the contrary, using timescales from molecular studies that suggested holosteans and teleosts evolved much earlier in Earth's history than when their first fossils appear, holosteans seemed to come out on top, appearing more innovative at evolving new sizes and faster at evolving between different shapes.

"There is no compelling evidence on any timescale that teleosts were the best at evolving different body sizes and shapes," said Clarke. "And in fact, if anything, there is some evidence hinting that maybe holosteans were more innovative when it came to evolving different body sizes and faster at changing shape."

The researchers also used the dataset to investigate whether genome duplication correlated with an increase in evolution rate and innovation. They found no consistent link with size evolution but did see indications that shape evolution was elevated in the more geologically recent teleosts with duplicate genomes relative to more ancient groups of teleosts. However, this occurred because those more ancient teleosts were particularly slow at evolving shapes since they compare equally poorly with holosteans, rather than signifying any exceptional evolution in those teleosts with duplicate genomes. On this basis, the authors deem the role of genome duplication on size and shape evolution to be "ambiguous," suggesting that, in agreement with recent studies of diversification in living teleosts, genome duplication is not the magic bullet that explains the diversity of all teleosts.

Clarke would like to continue delving into the history of neopterygian fishes, particularly those living fossils that are often neglected in favor of researching the more dynamic and diverse living teleosts.

"Many biologists have focused upon trying to explain why some groups are so incredibly successful," he said. "But there hasn't been a lot of focus on the other end of the spectrum: how do you get living fossils, these species-poor, long-lived groups that stick around doing the same thing for millions of years."