Biomedical bleeding affects horseshoe crab behavior

Each year, the U.S. biomedical industry harvests the blue blood from almost half a million living horseshoe crabs for use in pharmaceuticals -- most notably, a product called Limulus amebocyte lysate (LAL), used to ensure vaccines and medical equipment are free of bacterial contamination. This lifesaving product can only be made from horseshoe crab blood, says researcher Win Watson, UNH professor of zoology.

"The crabs are very heavily bled -- about 30 percent or more of their blood is taken, and that's a fair amount," says Chris Chabot, professor of neurobiology at PSU and a co-author on the study. "Approximately 20 to 30 percent of those crabs do not survive, so we were curious if any of the surviving crabs experienced nearly lethal effects from the bleeding," he said.

The study, "Sublethal Behavioral and Physiological Effects of the Biomedical Bleeding Process on the American Horseshoe Crab," was published recently in the journal The Biological Bulletin.

With funding provided by a N.H. Sea Grant development grant, Chabot, Watson and lead author Rebecca Anderson, a PSU graduate student, replicated the biomedical bleeding process to determine the potential impacts on the crabs' behavior.

After capturing the crabs in New Hampshire's Great Bay and transporting some of them back to Chabot's lab at PSU and others to UNH's Jackson Estuarine Laboratory, the researchers monitored their movements for a week and then harvested some of their blood. Electronic data recorders called accelerometers that measure the crabs' speed and direction were strapped to their backs and the crabs were placed in running wheels in tanks filled with seawater.

The researchers found that for approximately two weeks after the bleeding procedure, the crabs' behavior differed from their behavior prior to the bleeding -- they moved less frequently and with different patterns and rhythms, indicating they may have been disoriented. The researchers hope to track the movements of bled horseshoe crabs in their native environments to verify that their lab findings apply to real life behavioral changes.

One of the concerns, Anderson explains, is that the crabs are collected for bleeding during their breeding cycle when it's easiest to capture them on beaches. If the crabs are bled and then returned to the beach in a disoriented condition, they might be less likely to breed. This has the potential to exacerbate the population declines that are already occurring in parts of the east coast, including Delaware and Cape Cod. The populations in N.H. seem to be holding steady at the moment.

"If the biomedical industry could delay the blood harvest, it would probably help these animals," Chabot says. "For example, if they are bled after the breeding season, then they could recover in the subsequent months, fatten up and survive the winter without any problems to be able to breed again the following year," he said.

Watson suggests that the conditions under which they are transported could be improved as well. Fishermen capture the horseshoe crabs and often place them on a hot boat deck until they are transported by truck to the biomedical facility, all the while out of water and unable to breathe. If instead they were kept in cold, well-aerated water and perhaps fed, their stress levels might decrease and thus improve their chances of survival and ability to breed upon return to their native habitat.

Watson also notes that it is unclear which part of the process is the most detrimental -- the bleeding, the transporting, or simply keeping the crabs in the lab and out of their natural environment. Their research also revealed that the crabs' levels of hemocyanin, a protein in their system that helps transport oxygen in their blood, decreased for all individuals regardless of whether or not they were bled. Lowered hemocyanin levels might lessen the crabs' resilience to the stress of the experience.

Regardless, the researchers feel improvements in the collection and bleeding process are imperative to help preserve the species. In addition to the economic value of their blood, horseshoe crab eggs provide a critical food source for shorebirds that stop in Delaware on their migration to the Arctic. The adult crabs are also harvested for bait in the whelk and eel fisheries in many states, not including New Hampshire.

"We want to ensure that the biomedical bleeding is a sustainable practice, because horseshoe crabs are a critical component of the coastal ecosystem," Chabot says.

"I hope that this research helps to design management regulations that meet the needs of both the people and the crabs," Anderson adds.

The abstract for the article can be found online at: http://www.biolbull.org/content/225/3/137.abstract