Novel genomic screening tool enables precision reverse-engineering of genetic programming in cells

The researchers then perform single-cell analyses on each cell to measure the effects of the editing, including identifying which genes have been turned on or off and which genes are accessible (based on epigenetic markers). The team applied this tool to immature blood cells to identify the important transcription factors -- and DNA regions that code for them -- that strongly affect how blood cells develop.

They discovered that many of the DNA regions they identified as important for driving blood cell production are also regions known to harbor mutations linked to blood disorders. The DNA regions they identified as important occupy less than 0.3% of the genome, but they explain a disproportionately large share of the genetic influence on blood cell features and specialization.

In earlier work, investigators from this team and other collaborators used genome-wide association studies to identify the transcription factor responsible for switching off fetal hemoglobin after birth, laying the groundwork for the development of gene therapy for sickle cell disease and beta thalassemia.

This new Perturb-multiome approach enables researchers to systematically reveal how thousands of transcription factor variants influence blood cell production and influence disease risk, creating opportunities for finding many more novel targeted therapies for blood disorders.

Funding: La Caixa Foundation, the Rafael del Pino Foundation, the American Society of Hematology, the Broad Institute, the New York Stem Cell Foundation, the Lodish Family, the Howard Hughes Medical Institute, and the National Institutes of Health.