Unraveling Alzheimer's catalysts

Alzheimer's disease is characterized by the abnormal aggregation of Aβ into amyloid fibrils, which accumulate in the brain. Understanding the molecular mechanism of Aβ fibril formation is crucial in the fields of medicine and pharmacy. To address this, researchers focused on the interaction of Aβ with glycolipids called GM1 gangliosides on the neuronal cell membrane.

Using solid-state nuclear magnetic resonance spectroscopy and molecular dynamics simulations, the research group revealed that Aβ adopts a "U"-shaped conformation upon binding with GM1 gangliosides on the membrane surface. This "U"-shaped Aβ structure consists of two layers, the β1 layer (distal from the membrane) and the β2 layer (closer to the membrane), arranged alternately. In contrast to previously reported Aβ amyloid fibrils, which align in a uniform direction, the Aβ assembly on membranes containing GM1 gangliosides exhibited a completely different conformation. Notably, the highly exposed β1 layer on the membrane surface was found to act as a catalyst, significantly accelerating the fibrillation of surrounding Aβ molecules. Furthermore, the anti-GM1-Aβ antibodies were specifically observed to recognize this region.

This research successfully unveiled the three-dimensional structure of Aβ, acting as a catalytic platform for amyloid fibril formation, in the presence of GM1 gangliosides on neuronal cell membranes. While various therapeutic antibodies targeting Aβ aggregates have been developed, they primarily bind to amyloid fibrils or their precursors. The distinct Aβ structure discovered in this study offers novel possibilities as the anti-GM1-Aβ antibodies are capable of recognizing and binding to this unique conformation. Consequently, this research represents the first identification of the structural entity responsible for producing amyloid fibrils in brain tissue, potentially offering insights into predicting the onset risk of Alzheimer's disease and opening avenues for inhibiting its progression. The three-dimensional structure of Aβ molecules, as revealed in this study, provides exciting prospects for developing new therapeutic strategies against Alzheimer's disease.