Egg 'signatures' will allow drongos to identify cuckoo 'forgeries' almost every time, study finds

African cuckoos lay their eggs in drongos' nests to avoid rearing their chick themselves (an example of so-called brood parasitism). By forging drongo egg colours and patterns, cuckoos trick drongos into thinking the cuckoo egg is one of their own.

But drongos use knowledge of their own personal egg 'signatures' -- their eggs' colour and pattern -to identify cuckoo egg 'forgeries' and reject them from their nests, say scientists. These 'signatures' are like the signatures we use in our daily lives: unique to each individual and highly repeatable by the same individual.

Through natural selection, the African cuckoo's eggs have evolved to look almost-identical to drongo eggs -- a rare example of high-fidelity mimicry in nature.

A team led by researchers at the University of Cambridge and the University of Cape Town, working in collaboration with a community in Zambia, set out to explore the effectiveness of 'signatures' as a defence against highly accurate mimicry.

They found that despite near-perfect mimicry of fork-taileddrongo eggs, African cuckoo eggs still have a high probability of being rejected.

The findings are published today in the journal, Proceedings of the Royal Society B.

Researchers carried out field work in the Choma district of Zambia during September to November across four years. The first step was to measure the differences in colour and pattern of the fork-tailed drongo eggs and cuckoo eggs. The team found that the colour and pattern of cuckoo eggs was, on average, almost identical to that of drongo eggs, and that all the broad types of drongo egg 'signatures' were forged by the cuckoos.

"It is incredible how perfect the mimicry is. We have occasionally missed cuckoo eggs in the field because they looked exactly like the drongo clutch that they were found in," said lead researcher and Zoology PhD student Jess Lund.

The second step involved 'egg rejection' experiments in which the researchers simulated cuckoo visits by 'parasitising' drongo nests with foreign eggs from other drongo nests (as a proxy for African cuckoo eggs). They then checked the nest daily to see whether the drongo parents accepted the foreign egg as one of their own, or realised it was an imposter and rejected it by removing it from their nest. The team could then test what differences in colour and pattern between the foreign egg and the drongo's own eggs best predicted whether or not the drongo parents were tricked.

By combining results from both steps of the study, the researchers were able to create a model that predicted how often, on average, an African cuckoo would have its eggs rejected by a fork-tailed drongo host. They found the predicted rate of rejection to be 93.7%.

"We were surprised to see that so many of the cuckoo eggs were predicted to be rejected," said Lund "Our additional simulations show this is likely due to drongos having evolved 'signatures' on their eggs. Even though cuckoos have evolved excellent 'forgeries', individual cuckoos don't target individual drongo nests that match their own eggs. This means that for each cuckoo egg laid, the likelihood that it will be a good enough match to that drongo's 'signature' is very low."

Fork-tailed drongos have likely honed these 'signatures' and detection abilities through natural selection, explains Lund. "It's very costly for drongo parents if they don't have these skills. If they can't tell a 'forgery' from their 'signature' their own chicks will be killed by the cuckoo when it hatches, and they will be stuck raising a ravenous cuckoo for a whole breeding season."

The results of the study suggest that a female cuckoo may only fledge two chicks in her lifetime -- only just replacing herself and her mate. Researchers say that this would not amount to a sustainable population, which presents a puzzle because African cuckoos remain a common bird in many parts of Africa.

The researchers think that the fork-tailed drongos where the research took place could be particularly good at spotting 'forgeries'. "Perhaps this part of Zambia is a hotspot for parasitism, where drongos have particularly fine-tuned defences, and against which cuckoos stand little chance," said Lund.

The research was supported by the DST-NRF Centre of Excellence at the FitzPatrick Institute at the University of Cape Town, the BBSRC, the Royal Society, and the Department of Zoology at the University of Cambridge, and carried out with the crucial support of a community in Zambia who find and access drongo nests.