New mechanisms regulating plant response to temperature

"The clock is essential for plants to correctly respond to temperature stimuli," wrote first author Sorkin, and scientists have uncovered a variety of ways in which the circadian clock helps plants acclimate to changes in temperature and survive stress -- especially in model species like Arabidopsis. "The circadian clock in Arabidopsis is well-studied," mentioned Sorkin, "so the most exciting part of this project was finding a brand-new protein complex that regulates temperature responses. No one else had discovered this interaction, even in an established system." The complex comprises three proteins that interact in the evening to adjust to cooler temperatures. The research team importantly identified the mechanistic connection between these proteins and the specific time of days at which their interactions occur.

Sorkin went to heroic lengths to discover how these three protein 'puzzle pieces' come together," said Nusinow. "We are always looking for protein complexes in our work, but we don't know how they will interact. Maria's dedication solved that puzzle," he continued. Their findings are the result of three years of hard work -- sometimes at odd hours late at night and early in the morning -- to demystify how and when these proteins work together. Interestingly, the team "saw new complexes formed when we ran our experiments at different times of the day," Nusinow commented, "even just hours apart from each other."

The researcher's experiments involved collaboration with the Danforth Center's Proteomics & Mass Spectrometry Facility (PMSF) and plant growth team. The PMSF deployed state-of-the-art instrumentation to identify hundreds of potential proteins for the team to explore. In addition, collaborators from the University of Freiburg in Germany, the Plant-Environment Signaling Group at Utrecht University, and the Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas in Argentina, generously shared plant material for analysis of these proteins.

The Nusinow lab is excited to continue to study this protein complex at different temperatures with Stefanie King, a co-author and second-year graduate student at Washington University in St. Louis. "I'm grateful to learn from Maria and design experiments to look at the structure and regulation of the complex as a whole," King said. Now that the researchers demonstrated that the protein complex interacts at specific times of day, they are interested in further understanding the interaction under varying temperatures. In addition, Stefanie is looking forward to mentoring an NSF REU intern in these techniques over the summer.

This work was supported by the National Science Foundation, the National Institute of Health, the William H. Danforth Plant Science Fellowship at the Danforth Plant Science Center, the William H. Danforth Plant Science Fellowship from Washington University in St. Louis, the German Research Foundation, and the Agencia Nacional de Promoción Científica y Tecnológica.