Humans are leaving behind a 'frozen signature' of microbes on Mount Everest

According to new University of Colorado Boulder-led research, they're also leaving behind a frozen legacy of hardy microbes, which can withstand harsh conditions at high elevations and lie dormant in the soil for decades or even centuries.

The research not only highlights an invisible impact of tourism on the world's highest mountain, but could also lead to a better understanding of environmental limits to life on Earth, as well as where life may exist on other planets or cold moons. The findings were published last month in Arctic, Antarctic, and Alpine Research, a journal published on behalf of the Institute of Arctic and Alpine Research (INSTAAR) at CU Boulder.

"There is a human signature frozen in the microbiome of Everest, even at that elevation," said Steve Schmidt, senior author on the paper and professor of ecology and evolutionary biology.

In decades past, scientists have been unable to conclusively identify human-associated microbes in samples collected above 26,000 feet. This study marks the first time that next-generation gene sequencing technology has been used to analyze soil from such a high elevation on Mount Everest, enabling researchers to gain new insight into almost everything and anything that's in them.

The researchers weren't surprised to find microorganisms left by humans. Microbes are everywhere, even in the air, and can easily blow around and land some distance away from nearby camps or trails.

"If somebody even blew their nose or coughed, that's the kind of thing that might show up," said Schmidt.

What they were impressed by, however, was that certain microbes which have evolved to thrive in warm and wet environments like our noses and mouths were resilient enough to survive in a dormant state in such harsh conditions.

Life in the cryosphere

This team of CU Boulder researchers -- including Schmidt, lead author Nicholas Dragone and Adam Solon, both graduate students in the Department of Ecology and Evolutionary Biology and the Cooperative Institute for Research in Environmental Science (CIRES) -- study the cryobiosphere: Earth's cold regions and the limits to life in them. They have sampled soils everywhere from Antarctica and the Andes to the Himalayas and the high Arctic. Usually, human-associated microbes don't show up in these places to the extent they appeared in the recent Everest samples.

Schmidt's work over the years connected him with researchers who were headed to Everest's South Col in May of 2019 to set up the planet's highest weather station, established by the National Geographic and Rolex Perpetual Planet Everest Expedition.

He asked his colleagues: Would you mind collecting some soil samples while you're already there?

So Baker Perry, co-author, professor of geography at Appalachian State University and a National Geographic Explorer, hiked as far away from the South Col camp as possible to scoop up some soil samples to send back to Schmidt.

Extremes on Earth, and elsewhere

Dragone and Solon then analyzed the soil in several labs at CU Boulder. Using next-generation gene sequencing technology and more traditional culturing techniques, they were able to identify the DNA of almost any living or dead microbes in the soils. They then carried out extensive bioinformatics analyses of the DNA sequences to determine the diversity of organisms, rather than their abundances.

Most of the microbial DNA sequences they found were similar to hardy, or "extremophilic" organisms previously detected in other high-elevation sites in the Andes and Antarctica. The most abundant organism they found using both old and new methods was a fungus in the genus Naganishia that can withstand extreme levels of cold and UV radiation.

But they also found microbial DNA for some organisms heavily associated with humans, including Staphylococcus, one of the most common skin and nose bacteria, and Streptococcus, a dominant genus in the human mouth.

At high elevation, microbes are often killed by ultraviolet light, cold temperatures and low water availability. Only the hardiest critters survive. Most -- like the microbes carried up great heights by humans -- go dormant or die, but there is a chance that organisms like Naganishia may grow briefly when water and the perfect ray of sunlight provides enough heat to help it momentarily prosper. But even for the toughest of microbes, Mount Everest is a Hotel California: "You can check out any time you like/ But you can never leave."

The researchers don't expect this microscopic impact on Everest to significantly affect the broader environment. But this work does carry implications for the potential for life far beyond Earth, if one day humans step foot on Mars or beyond.

"We might find life on other planets and cold moons," said Schmidt. "We'll have to be careful to make sure we're not contaminating them with our own."

Additional authors on this publication include: Anton Seimon, Department of Geography and Planning, Appalachian State University; and Tracie Seimon, Wildlife Conservation Society, Zoological Health Program, Bronx, New York.

This work was supported by the National Geographic and Rolex Perpetual Planet Everest Expedition, the Department of Ecology and Evolutionary Biology, and the University of Colorado Boulder Libraries Open Access Fund