Neuropeptide discoveries could someday help defeat the dreaded cockroach

Neuropeptides are small proteins produced by neurons or endocrine cells that send messages to other cells. In insects, neuropeptides often act as neurotransmitters, hormones or growth factors, influencing an organism's development, growth, metabolism, behavior and reproduction. Therefore, disrupting these processes by targeting neuropeptides or their receptors is a potential new approach to pest control. Recently, Na Li and colleagues determined the genome sequence of P. americana. Now, they wanted to use this sequence, combined with peptide analysis, to characterize the neuropeptides of the American cockroach and study how their expression varies by tissue, developmental stage and sex.

The researchers searched the P. americana genome for genes predicted to encode neuropeptides and found 67 potential neuropeptide precursor genes. Then, they isolated peptides from four different cockroach tissues. Using mass spectrometry, the team identified 35 neuropeptides that were predicted by the genome sequence, as well as one new neuropeptide that hadn't been previously predicted or identified. Some of them were expressed in only a single tissue, whereas others were found in many tissues. Neuropeptide expression also varied between developmental stages and sexes, with most neuropeptides being more abundant in adult males. One neuropeptide, called sNPF, that was more highly expressed in females appeared to stimulate feeding behaviors, the researchers say, so it could be an attractive target for pest control measures.