Tiny marine organisms as the key to global cycles
New ways to understand the functioning of marine phytoplankton
- Date:
- April 6, 2020
- Source:
- Helmholtz Centre for Ocean Research Kiel (GEOMAR)
- Summary:
- Marine microorganisms play a very important role in global cycles such as of the uptake of carbon dioxide from the atmosphere. However, little is known about how they function. New approaches are for the first time laying the foundation for a more detailed genetic investigation of some key phytoplankton organisms.
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Marine microorganisms, such as bacteria and so-called protists, form a large part of the biomass in the oceans. Protists are a group of unicellular microorganisms that have a solid cell nucleus and are therefore different from bacteria. Many protists are part of the phytoplankton and as such, they drive important global processes. They perform photosynthesis and thus play an important role in removing CO2 from the atmosphere. They also form the basis of the food web by which marine fisheries are fueled. Yet little is known about how these microorganisms function. This is largely due to the fact that for a long time it was not possible to genetically modify key species of phytoplankton. However, this is precisely the methodology for studying the functioning of proteins that control many essential processes in organisms. Questions like 'How do fluctuations in phytoplankton growth occur?' or 'What underlies how different algae respond to seasonal changes in the ocean?' could be answered in this way.
In order to get to the bottom of these questions the Gordon and Betty Moore Foundation established a 'high-speed' international collaborative initiative for developing genetic systems in marine eukaryotic organisms. The study synthesis published today in Nature Methods brings together the results of scientists from 53 institutions and 14 countries. It was co-led by Alexandra Z. Worden (GEOMAR Helmholtz Centre for Ocean Research Kiel, Germany) alongside Julius Lukeš (Institute of Parasitology, Czech Academy of Science, Czech Republic) and Thomas Mock (University of East Anglia, UK).
Together, the international collaboration members worked to develop new ways of studying the individual proteins of marine protists. "The methods to analyze the function of individual proteins from key algal groups provide the scientific community with tools to carry out comparable investigations -- that connect to identifying factors that allow algae to thrive and to respond to environmental change," says Professor Worden.
In the publication, Worden's team presents manipulation protocols for the widespread alga Micromonas. This tiny cell is nearly all chloroplast, the organelle where photosynthesis occurs. It was first discovered in the 1950s when it was shown to be highly abundant in the English Channel -- and is now known for its worldwide distribution from pole to pole.
The concept behind the new methods is based on genetic transformation. This means that foreign DNA can be used to make proteins in an organism or that a certain gene in the organism can be switched off or removed. In many organisms, certain conditions have to be met for this transformation to occur. In order to induce it artificially, precise protocols must be followed. For many key species of marine protists like Micromonas, however, such protocols were not available -- until today's publication. During the project, the team developed procedures that successfully transformed 13 diverse marine protists, enabling a breadth of future studies on their ecology and evolution.
"The new findings will allow us to understand the mechanisms algae use to respond to changes in the environment. This concerns understanding seasonal fluctuations due to a natural, annual rhythm, as well as transitions related to climate change," adds Worden.
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Materials provided by Helmholtz Centre for Ocean Research Kiel (GEOMAR). Note: Content may be edited for style and length.
Journal Reference:
- Drahomíra Faktorová, R. Ellen R. Nisbet, José A. Fernández Robledo, Elena Casacuberta, Lisa Sudek, Andrew E. Allen, Manuel Ares, Cristina Aresté, Cecilia Balestreri, Adrian C. Barbrook, Patrick Beardslee, Sara Bender, David S. Booth, François-Yves Bouget, Chris Bowler, Susana A. Breglia, Colin Brownlee, Gertraud Burger, Heriberto Cerutti, Rachele Cesaroni, Miguel A. Chiurillo, Thomas Clemente, Duncan B. Coles, Jackie L. Collier, Elizabeth C. Cooney, Kathryn Coyne, Roberto Docampo, Christopher L. Dupont, Virginia Edgcomb, Elin Einarsson, Pía A. Elustondo, Fernan Federici, Veronica Freire-Beneitez, Nastasia J. Freyria, Kodai Fukuda, Paulo A. García, Peter R. Girguis, Fatma Gomaa, Sebastian G. Gornik, Jian Guo, Vladimír Hampl, Yutaka Hanawa, Esteban R. Haro-Contreras, Elisabeth Hehenberger, Andrea Highfield, Yoshihisa Hirakawa, Amanda Hopes, Christopher J. Howe, Ian Hu, Jorge Ibañez, Nicholas A. T. Irwin, Yuu Ishii, Natalia Ewa Janowicz, Adam C. Jones, Ambar Kachale, Konomi Fujimura-Kamada, Binnypreet Kaur, Jonathan Z. Kaye, Eleanna Kazana, Patrick J. Keeling, Nicole King, Lawrence A. Klobutcher, Noelia Lander, Imen Lassadi, Zhuhong Li, Senjie Lin, Jean-Claude Lozano, Fulei Luan, Shinichiro Maruyama, Tamara Matute, Cristina Miceli, Jun Minagawa, Mark Moosburner, Sebastián R. Najle, Deepak Nanjappa, Isabel C. Nimmo, Luke Noble, Anna M. G. Novák Vanclová, Mariusz Nowacki, Isaac Nuñez, Arnab Pain, Angela Piersanti, Sandra Pucciarelli, Jan Pyrih, Joshua S. Rest, Mariana Rius, Deborah Robertson, Albane Ruaud, Iñaki Ruiz-Trillo, Monika A. Sigg, Pamela A. Silver, Claudio H. Slamovits, G. Jason Smith, Brittany N. Sprecher, Rowena Stern, Estienne C. Swart, Anastasios D. Tsaousis, Lev Tsypin, Aaron Turkewitz, Jernej Turnšek, Matus Valach, Valérie Vergé, Peter von Dassow, Tobias von der Haar, Ross F. Waller, Lu Wang, Xiaoxue Wen, Glen Wheeler, April Woods, Huan Zhang, Thomas Mock, Alexandra Z. Worden, Julius Lukeš. Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods, 2020; DOI: 10.1038/s41592-020-0796-x
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