New temperature sensing mechanism in plants

In a paper published in Nature Communications, a group of cell biologists led by Meng Chen, a professor of botany and plant sciences at the University of California, Riverside, reveal the phytochrome B molecule has unexpected dynamics activated by temperature, and behaves differently depending on the temperature and type of light.

As climate change warms the world, crop growth patterns and flowering times will change. A better understanding of how phytochromes regulate the seasonal rhythms of plant growth will help scientists develop crops for optimal growth under the Earth's new climate and might even shed light on cancer in animals.

Phytochromes switch between active and inactive forms like a binary switch controlled by light and temperature. In direct sunlight, such as in open fields, phytochromes switch "on," absorbing far-red light. This active form inhibits stem elongation, which limits how tall plants in direct sunlight can grow.

In shade phytochromes are less active, absorbing red. This "off" form releases the inhibition of stem growth, so plants grow taller in shade to compete with other plants for more sunlight.

Within the cell, light causes "on" phytochromes to coalesce into units called photobodies inside the cell nucleus. When phytochrome B is off, it resides outside the cell nucleus. It moves inside the nucleus when "on" and changes the expression of genes and growth patterns.

Changes in light alter the size and number of all foci. Chen's group has now shown temperature alters individual foci.

His group examined the behavior of cells exposed to different temperature and light conditions from the leaves and stems of Arabidopsis thaliana, a plant used as a standard model in botany science. The goal was to monitor how photobodies change in response to temperature.

The current understanding is that phytochromes form photobodies only in the "on" state.

Chen and co-authors Joseph Hahm, Keunhwa Kim, and Yongjian Qiu, members of Chen's research group at UC Riverside, expected increasing the temperature would have a similar effect to shade -- it would switch the phytochromes "off." They thought the photobodies would disappear, as in shade.

The results were completely unexpected.

The team found increasing the temperature did not cause all the photobodies to disappear at once. Instead, specific photobodies disappeared in specific ranges of temperature. Increasing the temperature incrementally reduced the number of photobodies as they disappeared selectively.

"We found that a subset of thermostable photobodies can persist even in warm temperatures," Chen said. "The rest of the foci would disappear at each stage of lower temperature. Before we thought all the foci were the same, but now we know they are all different."

The mechanism that makes them disappear selectively must be different from the mechanism that makes them disappear in shade. This suggests individual photobodies could be sensors for specific temperature ranges.

The study also showed phytochrome B reacts to temperature from two different locations on the molecule. The first part senses temperature; the second part forms foci. Foci formed by this second location are insensitive to temperature. This shows light and temperature are sensed by the same part of the molecule but result in different behaviors.

"Photobodies are large, dynamic protein complexes. Our results suggest that each of them could have a different composition," Chen said. "What we think is that the unique composition of individual photobodies make them react to temperature differently. Future studies on understanding the unique features of each photobody will likely uncover the underlying mechanisms of temperature sensing and the regulation of temperature-responsive gene expression in plants."

In addition to helping develop plants that will thrive in a warming world, this work could help scientists learn more about cancer in animals. Proteins in animal cells also form foci related in some way to cancer, but their role in gene expression and regulation is unknown.