Tau protein suppresses neural activity in mouse models of Alzheimer's disease

"Our current study reinforces growing evidence suggesting that A-beta and tau work together to impair brain function and that, for certain aspects of that impairment, tau predominates," says Bradley Hyman, MD, PhD, director of the Alzheimer's Unit at the MassGeneral Institute for Neurodegenerative Disease, senior author of the paper. "We are intrigued to learn how they are interacting at a molecular level, in order to find ways of blocking that synergy."

The team used advanced multiphoton calcium imaging to measure neural activity is several mouse models of Alzheimer's disease. Experiments in a model that overexpresses the human form of A-beta confirmed previous studies that the plaques lead to hyperactivity of neurons. Studies with two mouse models that overexpress different forms of tau found, for the first time, that elevated levels of the protein were associated with a significant reduction in neural activity whether or not tau had aggregated into tangles.

Experiments with a novel mouse model that overexpresses both A-beta and tau found that, in the presence of both pathological proteins, A-beta-associated hyperactivity was abolished and tau's neuronal silencing effect predominated. The findings were duplicated in mice regardless of their age, including animals too young to exhibit the loss of neurons typically seen in animals that only overexpress tau.

Since the mouse models used in this study are uniquely engineered so that tau expression can be blocked by administration of the antibiotic drug doxycycline, the researchers measured neural activity in the tau-overexpressing mice and in the animals that overexpressed A-beta and tau both before and six weeks after doxycycline administration. While blocking tau expression returned neural activity to normal levels in the mice that overexpressed that protein only, doxycycline treatment did not restore neural activity in animals expressing both pathologic proteins, even in mice too young to show neural damage.

The authors note that their findings could help explain why clinical trials of A-beta-blocking therapies have had difficulty improving symptoms of patients with Alzheimer's disease. "One implication of our work is that approaches combining anti-A-beta and anti-tau therapies might be more effective than either alone, at least from the perspective of neural activation," says Hyman, the Penney Professor of Neurology at Harvard Medical School. "Finding that tau and A-beta work in a synergistic fashion opens the doors to new research into understanding exactly how that interaction works."