New models could uncover important answers for Alzheimer's researchers

"One of the defining traits of Alzheimer's is the progressive accumulation of amyloid-β plaques in the brain," said Yuksel Agca, associate professor of veterinary pathology and a researcher in the Comparative Medicine Program in the MU College of Veterinary Medicine. "Two proteins, APP and PS1 are linked to the development of Alzheimer's disease in humans -- and these two targets have become the basis for numerous studies. If we can identify how to manipulate amyloid-β build up, we can reduce the production of harmful plaques in the brain, leading to decreases in the onset of Alzheimer's disease."

The MU researchers developed rats that had human APP and PS1 proteins. The rats then underwent behavioral assessments in the Barnes maze. The maze, which tracks rodent behavior is used to measure learning and spatial memory. In a series of experiments, the Alzheimer's model rats that produced human APP and PS1 displayed poor memory and learning.

After the maze experiments, the research team assessed amyloid-β levels through serum tests, as well as brain screenings, which showed vascular changes and amyloid-β plaques similar to the ones observed in humans suffering from Alzheimer's disease.

"We found that these rat models could be useful in the fight against Alzheimer's in people," Agca said. "Because of their shorter lifespans and their larger size, translational models such as rats are extremely helpful in ongoing studies of disease. The results can be translatable to humans in identifying targets for drugs as well as identifying everyday lifestyle changes we can make to help stave off disorders like Alzheimer's. We hope this model will become beneficial as research continues to move forward."

Just like plaque on teeth or in arteries, plaque buildup in the brain can be decreased in people by maintaining a good diet and exercise, Agca said.

The study, "Memory deficiency, cerebral amyloid angiopathy, and amyloid-β plaques in APP+PS1 double transgenic rat model of Alzheimer's disease," was published in PLOS ONE. This study was supported by the University of Missouri-Research Incentive Funds to Yuksel Agca and the National Institutes of Health (# P40 OD011062). Diana Klakotskaia, a graduate student in the Department of Psychological Science in the MU College of Arts and Science; and Cansu Agca, a researcher in the Department of Veterinary Pathobiology contributed to the study. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies.