Optical tools to detect metabolic changes linked to disease

The method is based on the fluorescence of two important coenzymes (biomolecules that work in concert with enzymes) when excited by a laser beam. The coenzymes -- nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) -- are involved in a large number of metabolic pathways in every cell. To find out the specific metabolic pathways affected by disease or stress, the Tufts scientists looked at three parameters: the ratio of FAD to NADH, the fluorescence "fade" of NADH, and the organization of the mitochondria as revealed by the spatial distribution of NADH within a cell (the energy producing "batteries" of the cell).

The first parameter -- the relative amounts of FAD to NADH -- can reveal how well the cell is consuming oxygen, metabolizing sugars, or producing or breaking down fat molecules. The second parameter -- the fluorescence "fade" of NADH -- reveals details about the local environment of the NADH. The third parameter -- the spatial distribution of NADH in the cells -- shows how the mitochondria split and fuse in response to cellular growth and stress.

"Taken together, these three parameters begin to provide more specific, and unique metabolic signatures of cellular health or dysfunction," said Irene Georgakoudi, Ph.D., corresponding author of the study and a professor of biomedical engineering in the School of Engineering at Tufts. "The power of this method is the ability to get the information on live cells, without the use of contrast agents or attached labels that could interfere with results."

Other methods exist for non-invasively tracking the metabolic signatures of disease, such as the PET scan, which is often used in research. But while PET scans provide low resolution information with excellent depth penetration into living tissues, the optical method introduced by the Tufts researchers detects metabolic activity at the resolution of single cells, although mostly near the surface.

That is not necessarily a limitation. Many diseases can be detected at the surface of tissues, including cancer, while many pre-clinical studies are performed with animal models and engineered three-dimensional tissues that can benefit from being monitored non-destructively. The method developed by Georgakoudi and colleagues may prove to be a powerful research tool for understanding their metabolic signatures.