Using wild rice to predict health of Minnesota lakes and streams

Sulfate is a compound that is released into fresh water from mining, sewage, fertilizers, fossil fuel combustion, and other human activity, as well as from natural geological sources in some parts of the state. The researchers recently published three related studies on the topic of sulfate in water in the Journal of Geophysical Research: Biogeosciences, published by the American Geophysical Union.

"Wild rice serves as a flagship species or the equivalent of our 'canary in a coal mine,' giving us a warning on how human activity affects our water quality in Minnesota," said Amy Myrbo, a research associate in the University of Minnesota's LacCore/CDSCO facility in the Department of Earth Sciences and lead author of two of the three studies. "The results of our studies are important because they show that increases in sulfate in our lakes and streams can have multiple negative consequences for ecosystems, even though sulfate itself is relatively benign."

Wild rice is culturally important to multiple groups in Minnesota, especially Native Americans. Wild rice also provides habitat and food for waterfowl and other wildlife. Research in the 1940s and 1950s found that wild rice grew best in low-sulfate Minnesota lakes, but no one knew why. The correlation was a puzzle, since sulfate isn't very toxic to plants or animals.

This new research finds that the problem is sulfide, not sulfate. Sulfate can be converted into toxic levels of sulfide in the soil of wetlands, like those in which wild rice germinates and roots. Wild rice is an annual plant that must sprout each spring from seeds that were dropped the previous fall into the wet soil. Anaerobic microbes in the soil make sulfide from sulfate in the overlying water. Lakes, streams, and wetlands that have high concentrations of dissolved sulfide in the sediment therefore have a low probability of hosting wild rice.

In addition to the release of sulfide, the researchers found that accelerated decomposition of plants in waterways caused by the sulfates releases phosphorus and nitrogen, which fertilizes the waterbody and can change the plant community within the ecosystem -- including increasing algae blooms. The microbes that convert sulfate to sulfide also produce methylmercury, the only form of mercury that contaminates fish.

"Minnesota is unique among U.S. states and Canadian provinces in that it has a water quality standard that regulates sulfate," Myrbo said. "This isn't just about wild rice. We've now found that putting sulfate into our water has consequences down the line, including more mercury in fish, changes in habitat for ducks, and changes in the food chain."

Over three summers in 2011, 2012, and 2013, researchers studied water samples and sediment from more than 100 lakes and streams in Minnesota where wild rice grows or where they determined wild rice should be growing. Many of these waterways were in northeastern Minnesota where sulfate does not naturally occur. The researchers did more than 100 measurements at every site studying sediment cores, water in the sediment, surface water, and various plants. They analyzed things like the water's chemistry, depth, and transparency.

In almost all cases, the water with the highest levels of sulfate had no wild rice, even though other conditions seemed ideal for the growth of the aquatic plant. The team's field research that showed the effects of sulfate on water quality mirrored studies by other researchers in tanks.

"Our research is based on hard science and showed the same results as other studies in artificial environments," Myrbo said. "We know that issues regarding our waterways can be very complex, but we hope our work can be used to make good public policy that balances the economic needs of our state with food sovereignty and protecting the environment."

In addition to Myrbo, researchers involved in the study include Curt Pollman, Aqua Lux Lucis, Inc. in Gainesville, FL; Ed Swain, Minnesota Pollution Control Agency; Nathan Johnson, Department of Civil Engineering, University of Minnesota-Duluth; John Pastor, Department of Biology, University of Minnesota-Duluth; Marta Shore, School of Public Health, University of Minnesota-Twin Cities; and several other partners from the St. Croix Watershed Research Station of the Science Museum of Minnesota, University of Wisconsin River Falls, and Minnesota Department of Health, as well as 20 University of Minnesota students and employees who were part of the field crew.

The research was funded by the State of Minnesota Clean Water Fund, created by the Clean Water, Land and Legacy Amendment to Minnesota's constitution, and the National Science Foundation through the University of Minnesota LacCore Facility.

"We feel that this is just the beginning of our research on studying Minnesota's waters and the effects on communities," Myrbo said.

Myrbo and University colleagues recently received a $720,000 University of Minnesota Grand Challenges research award for new research on using wild rice as a flagship for assuring clean water and equitable communities.