First-time reconstruction of infectious bat influenza viruses

Now a team at the Institute for Virology of the Medical Center -- University of Freiburg, Germany, together with scientists from Switzerland and the USA, report a break-through in isolating a bat influenza virus by reconstituting fully functional bat influenza viruses in the laboratory. Unexpectedly, the bat influenza viruses infected not only bat cells but also dog and human cells. A corresponding study was published online on October 24, 2016, in the journal Proceedings of the National Academy of Sciences.

Reconstructed bat influenza virus is essential for risk assessment.

A first step in generating bat influenza A-like viruses was to select cell lines susceptible to infection. Such cells needed to express receptors on the cell surface for bat virus attachment and entry. The research teams analyzed over 30 cell lines from different species for their capacity to recognize and internalize bat influenza viruses. They did this by first using another virus, vesicular stomatitis virus, which can infect many different cell types. The trick was that they engineered this virus to incorporate on its own surface a bat influenza virus protein that the bat virus normally uses for entry into cells. Then the cell line most susceptible to the engineered vesicular stomatitis virus was used to reconstruct the original bat influenza A-like virus, starting out with the known influenza-like viral genome sequences isolated from the bats. "Infectious bat viruses were readily obtained. Interestingly, most cells, including human cells, that supported infection with the vesicular stomatitis indicator virus were also susceptible to direct infection with the reconstructed bat influenza virus," explains Prof. Martin Schwemmle, group leader at the Institute for Virology at the Medial Center -- University of Freiburg.

The new observation that human cells can be infected with bat influenza A-like viruses is a hint that these viruses could also potentially be transmitted to humans. Although there is no evidence yet for such transmissions, the new findings are a wake-up call for more research. "It is too early to jump to definite conclusions on the ability of these virus to cross the species barrier and infect humans but our studies make it possible now to conduct further experiments and analyze the risk that these viruses pose for other species," says Prof. Schwemmle.