Plants get a brace to precisely shed flowers and leaves

In order for leaves, flowers and fruits to drop, specific proteins, known as cell wall processing enzymes, need to precisely act to degrade the cell wall between the cells, facilitating the cell-to-cell detachment. Similar to a plant wound, the opening must be quickly re-sealed to avoid access of bacteria and harmful substances, but in comparison to a wound, the team found out that plants use a different mechanism to seal the opening.

The detachment spot (abscission zone) was found to consist of two neighboring cell types: residuum cells (RECs) remaining on the plant and secession cells (SECs) in flowers, leaves, etc. "The separation must be precise and minimal as the process transiently increases vulnerability to environmental peril," explains June M. Kwak, leading author of the study.

DGIST and IBS researchers used the classic plant model, Arabidopsis thaliana, to investigate how plants overcome the physical constraint of cell walls, and accomplish organ abscission. The two cell types in the abscission zone display different cellular activities and architectures. In particular, SECs form two to three layers of lignin with a honeycomb structure. On the detachment area, lignin acts as a molecular brace that may hold individual SECs together when they shed from the plant. In addition, it might limit cell wall processing enzymes to a confined area, allowing precision abscission.

"Because of the light and rigid property of the honeycomb architecture, it is perhaps unsurprising that we find plant cells have evolved such a structure to accomplish a potentially dangerous cellular process with great accuracy," explains Kwak.

Immediately after SECs are gone with the wind, RECs accumulate a thin cell wall, which is vulnerable to infections and external harm. To protect the newly formed cell surface, RECs shield themselves with a protective coating made of cutin, which is responsible for the surface protection against pathogens. This indicates that non-epidermal RECs change identity turning into epidermal cells. This is particularly interesting as the specification of epidermal cell identity was thought to be restricted to the embryonic stage.

The formation of this cutin layer is distinct from the mechanism of plant wound healing. The latter provides a protecting barrier made of cork rather than cutin. How the two processes are related is still mysterious. And while looking for the answer, let's enjoy the beautiful blooming of flowers and change of seasons.