Life history of the 360-million-year-old tetrapod Acanthostega rewrites the tetrapod move to land

The tetrapods are four-limbed vertebrates, which are today represented by amphibians, reptiles, birds and mammals. Early tetrapods of the Devonian period (419-359 million years ago) are of great interest to palaeontologists: they were the earliest vertebrate animals that ventured onto land, paving the way for all future vertebrate life on land. The move from water to land must have affected every aspect of the biology of these animals, but until now there has been no serious attempt to investigate their life histories -- how long they lived or whether they had an aquatic juvenile stage, for example. Well-preserved skeletons are rare and it has simply been assumed that they represent adults.

The single richest locality for Devonian tetrapods is a so-called mass-death deposit of Acanthostega, discovered in 1987 in Greenland by Jennifer Clack, one of the authors of the study and leading teams from the Universities of Cambridge and Copenhagen, where dozens of skeletons lie packed together like sardines in a tin. It looks like the tetrapods all died together when a small stream within an "inland delta" (like the modern Okavango in Botswana) dried out. The team decided to look at the life history of these fossils by investigating the internal structure of their humeri (upper arm bones). "Using the tremendous power of synchrotron X-rays, we were able to access microscopic details in these dense specimens as on real histological slices, but without damaging these unique fossils" says Paul Tafforeau from the ESRF.

The microscopic structures in the bones of these fossil tetrapods are almost perfectly preserved. "Like a growing tree, a limb bone is marked by seasonal rhythms and lays down annual growth rings" says Sophie Sanchez, the lead author of the publication, working at Uppsala University and the ESRF. "These growth rings, which can be seen in both fossil and living tetrapods, are informative about the development and age of the individual."

The powerful X-ray beam of the ESRF revealed that all studied fossils of Acanthostega were immature individuals, even though they were at least 6 years old and probably older. Their growth had not yet begun to slow down as it does at sexual maturity. In addition, the researchers showed that Acanthostega's foreleg remained cartilaginous until late during its development.

In contrast to bone, cartilage is a non-mineralised tissue, elastic and far too weak to allow the forelegs to sustain the weight of the animal's body out of the water. "This suggests that the Acanthostega mass-death deposit represents a school of aquatic juveniles that included few or no adults" says Per Ahlberg from Uppsala University. So where were the adult Acanthostega living? Were there segregated distributions of juveniles and adults at least at certain times? This remains to be discovered. The scans done at ESRF ID19 beamline also show that the absolute size at which limb ossification began differs greatly between individuals, suggesting the possibility of sexual dimorphism, adaptive strategies or competition-related size variation.

The tetrapods' move onto land was arguably one of the most radical adaptive shifts in vertebrate evolutionary history. "Our study provides a first glimpse of the life-history traits of an early tetrapod. We plan to undertake a more complete survey of early-tetrapod life histories which should have a significant impact on theories depicted in all textbooks" concludes Sophie Sanchez.

This research was supported by an ERC grant and a grant from the Vetenskapsradet.