Plant defense as a biotech tool

What is useful for the plants, is also in demand in industry where the reverse reaction of the HNL-enzymes allows to bind cyanide to different molecules. This creates a double benefit. On the one hand, it is possible to recycle unwanted cyanide wastes, which for example are generated during the production of acrylonitrile. Acrylonitrile is not only used in adhesives, it is also the raw material for polyacrylonitrile or "acrylic," an important fiber for textiles. On the other hand, industry gains valuable building blocks for pharmaceutical agents or the vitamin synthesis. The extremely high specificity of the HNL-enzymes makes them so useful for industrial application. Ideally, through biocatalysis valuable products are derived from inexpensive precursors.

HNL-enzymes have a fine tradition in industry. The first HNL enzymes have been successfully developed in the mid-1990s at Graz University of Technology (TU Graz) and were used industrially for the production of insect repellents. Important improvements in the synthesis of high-value products aroused more industrial interest. The early enzymes certainly can't meet all of today's requirements, so researchers are searching for new HNL biocatalysts. Within the framework of the EU project KYROBIO, which deals with production technologies for new molecules, acib-researchers have successfully looked for those bio-tools.

Smell enzyme activity

The acib-researchers Margit Winkler, Elisa Lanfranchi and Anton Glieder finally made a find in the white rabbit's foot fern, where the scientists sniffed enzyme activity. "When you rub a young fern croizer between the fingers, it smells of hydrocyanic acid and benzaldehyde (similar to marzipan), indicating that there is enzyme activity," explains Margit Winkler, "knowing that ferns show the desired activity, we have been searching in the woods and in commercially available plants." In three and a half years the acib-researchers in Graz have kept a close eye on eligible enzymes, examined their structure, produced the biocatalysts biotechnologically and tested their activity. Finally, the enzymes of a commercial rabbit's-foot fern from the hardware store were the most promising. The Styrian bracken fern also showed activity and is currently being studied in more detail.

The new enzyme has an extremely high activity, although it is not even optimized. "Our new HNL is more efficient and simpler to handle than those previously used, because it is a small, uncomplicated enzyme," says Anton Glieder (TU Graz, acib). These results are a perfect basis for the industrial utilization. The range of applications is huge: It includes everything from crop protection to the production of repellents against mosquitoes and Co.

The acib-method used for bioprospecting of enzyme activities was published in the Current Biotechnology.