iPS cells discover drug target for muscle disease

The strategy depends on first reprogramming the patient cells to induced pluripotent stem (iPS) cells and then introducing a gene that differentiates the iPS cells to muscle cells. "Our model allows us to use the same genetic background to study the early stage of pathogenesis which was not possible in the past," says CiRA researcher Emi Shoji, the first author of a study published in Scientific Reports that gives new insights on DMD development.

Muscle contraction depends on an influx of calcium (Ca2+) ions into the cells. However, too much influx leads to cell dysfunction or death, which is believed to be the underlying cause of DMD. "It is critical to assess intact cells for an accurate evaluation how Ca2+ influx leads to DMD pathogenic cascades," says Shoji. She therefore stimulated their model electrically to simulate muscle cell contraction, finding that cells from DMD patients had significantly increased influx. Further study laid blame on transient receptor potential (TRP) channels through which the Ca2+ ions enter the cell. This observation is consistent with other models and provides a clear drug target for the treatment of DMD. More important, it should allow scientists to uncover drug agents that can counter DMD at early development. "TRP channels have been identified before. But because our model uses patient-derived hiPS cells, there is a potential that we can find new drugs for DMD," says Sakurai.