Earth organisms survive under Martian conditions: Methanogens stay alive in extreme heat and cold

Methanogens, microorganisms in the domain Archaea, use hydrogen as their energy source and carbon dioxide as their carbon source, to metabolize and produce methane, also known as natural gas. Methanogens live in swamps and marshes, but can also be found in the gut of cattle, termites and other herbivores as well as in dead and decaying matter.

Methanogens are anaerobic, so they they don't require require oxygen. They don't require organic nutrients and are non-photosynthetic, indicating they could exist in sub-surface environments and therefore are ideal candidates for life on Mars.

Rebecca Mickol, a doctoral student in space and planetary sciences at the University of Arkansas, subjected two species of methanogens to Martian conditions: Methanothermobacter wolfeii and Methanobacterium formicicum. Both species survived the Martian freeze-thaw cycles that Mickol replicated in her experiments.

The species were tested for their ability to withstand Martian freeze-thaw cycles that are below the organisms' ideal growth temperatures: 37 degrees Celsius (98.6 degrees Fahrenheit) for M. formicicum and 55 degrees Celsius (131 degrees Fahrenheit) for M. wolfeii.

"The surface temperature on Mars varies widely, often ranging between minus 90 degrees Celsius and 27 degrees Celsius over one Martian day," Mickol said. "If any life were to exist on Mars right now, it would at least have to survive that temperature range. The survival of these two methanogen species exposed to long-term freeze/thaw cycles suggests methanogens could potentially inhabit the subsurface of Mars."

Mickol conducted the study with Timothy Kral, professor of biological sciences in the Arkansas Center for Space and Planetary Sciences and lead scientist on the project. She is presenting her work at the 2014 General Meeting of the American Society for Microbiology, being held May 17-20 in Boston.

The two species were selected because one is a hyperthermophile, meaning it thrives under extremely hot temperatures, and the other is a thermophile, which thrives under warm temperatures.

"The low temperature on Mars inhibited their growth, but they survived," Mickol said. "Once they got back to a warm temperature, they were able to grow and metabolize again. I wanted to see if these cold temperatures would kill them, or if they were able to survive and adapt."

Since the 1990s, Kral has been studying methanogens and examining their ability to survive on Mars. In 2004, scientists discovered methane in the Martian atmosphere, and immediately the question of the source became an important one.

"When they made that discovery, we were really excited because you ask the question 'What's the source of that methane?'" Kral said. "One possibility would be methanogens."

Mickol is currently interning at the Kennedy Space Center in Florida. The NASA Exobiology Program funded her research.