Sponge shines light on life's origin: Genome connects the dots between Amphimedon, animal descendants

In a paper published online in the journal Nature, Rice University's Nicholas Putnam is among a group of scientists who have established a draft genome sequence for Amphimedon queenslandica, a sponge found off the coast of Australia. The genome is helping evolutionary biologists connect the dots as they look for DNA sequences shared by metazoans, or multicelled animals.

Sponges are an ancient group, with fossils dating back at least 650 million years. They are thought to have been the first group of animals to branch from all the others. Therefore, genes shared by sponges and other animals must have been present in the common ancestor of all metazoans. This ancestor would have evolved mechanisms to coordinate cell division, growth, specialization, adhesion and death; this suggests that early sponges already had a developmental set of tools similar to those in metazoans today, said Putnam, an assistant professor of ecology and evolutionary biology.

"What's exciting is the new things we're learning about animal evolution," said Putnam, who got involved with the project while working at the Department of Energy's Joint Genome Institute in 2006. "For example, sponges have embryos, and having the genome helps us look at how they develop and make specific connections to developmental pathways in other animals.

"It's the kind of thing that will lead to a much clearer understanding of what the very first metazoans looked like," he said.

That distant ancestor may well have looked like a sponge. For the paper, Putnam helped compare Amphimedon's draft genome with 13 other complete animal genomes, including a selection of invertebrates, as well as a choanoflagellate. The researchers wrote of a "striking conservation of gene structure and genome organization" that is common to all. "We can now say that the large-scale patterns of genome organization we've seen conserved in other animal groups come from the very root of the animal tree," Putnam said.

The challenge ahead is learning what they do. "The focus of my research is to understand whether patterns that have been around for a billion years have some particular functions -- or if they're hanging around because not enough time has gone by to erase them."

What's missing is also interesting, he said. The ancestral patterns of genome organization common to other creatures is absent from certain arthropods -- invertebrates that include the likes of centipedes and lobsters -- and nematodes. "If the missing pattern is neutral, you'd say that somewhere along the history of those groups, the rate of (evolutionary) change sped up enough to break the connection," Putnam said. "If it's functional, then somehow those groups overcame whatever constraint is on it in other lineages."

Also puzzling is that while Amphimedon shares key developmental genes with a diverse set of metazoans, its basic structure hasn't changed in 600 million years. Given the same roots, researchers wonder why it didn't evolve more radically, and they are working to identify the differences that gave rise to, say, nerve cells in other creatures but not sponges.

Unlocking the basic mechanisms of multicellularity may also help researchers understand what happens when those mechanisms go wrong and lead to cancer and autoimmune disorders.

The paper's senior authors are Daniel Rokhsar of the University of California, Berkeley, and Bernard Degnan of the University of Queensland in Australia.

The work was funded by the Australian Research Council, the Department of Energy Joint Genome Institute, Harvey Karp, the National Science Foundation, the National Institutes of Health/National Human Genome Research Institute, the University of Queensland Postdoctoral Fellowship, the Sars International Center for Marine Molecular Biology, Deutsche Forschungsgemeinschaft, Agricultural and Natural Resources/University of California, the French National Center for Scientific Research, the Gordon and Betty Moore Foundation and Richard Melmon.