Tiny Laser-scanning Microscope Images Brain Cells In Freely Moving Animals

The research is published Nov. 2 in the Proceedings of the National Academy of Sciences.

The majority of our life is spent moving around a static world and we generate our impression of the world using visual and other senses simultaneously. It is the ability to freely explore our environment that is essential for the view we form of our local surroundings. When we walk down the street and enter a shop to buy fruit, the street, shop and fruit are not moving, we are. What our brain is probably doing is constantly updating our position based on the information received from our sensory inputs such as eyes, ears, skin as well as our motor and vestibular systems, all in real time. The problem for researchers trying to understand how this occurs has always been how to record meaningful signals from the brain cells that do the calculations while we are in motion.

To get around this problem researchers at the Max Planck Institute for Biological Cybernetics in Tübingen have developed a way of actually watching the activity of many brain cells simultaneously in an animal that is free to move around the environment. By developing a small, light-weight laser-scanning microscope, researchers were able to, for the first time, image activity from fluorescent neurons in animals that were awake and moving around, while tracking the exact position of the animal in space. The microscope uses a high-powered pulsing laser and fiber optics to scan cells below the surface of the brain, eliminating the need to insert electrodes, which are traditionally used. Because of this, the microscope is non-invasive to the brain tissue.

The traditional approach to solving these sorts of questions is to restrain the animal and present it with a series of scenes or movies or images. The miniaturized microscope allows the researchers to turn this paradigm around and allow the animal to freely move around in its environment, while still allowing the scientists to monitor the activity of the brain cells responsible for processing visual information. It is clear that the brain does not work one cell at a time to recognize the environment, so the microscope records from many cells at a time, allowing for the first time the ability to look at how the brain is able to generate an internal representation of the outside world, while using natural vision.

"We need to let the animal behave as naturally as possible if we want to understand how its brain operates during interaction with complex environments. The new technology is a major milestone on the way to helping us understand how perception and attention work," says Jason Kerr, lead author of the study.