Digital enhancement of cryoEM photographs of protein nanocrystals

The procedure paves the way towards full three-dimensional structure determination at high resolution for protein crystals. The authors report on how lattice information can be enhanced by means of a wave finder in combination with Wiener-type maximum-likelihood filtering. The lattice filter is a very powerful tool for selecting and analysing extremely low contrast cryo-images of three dimensional protein/peptide nanocrystals. It confirms that the three-dimensional crystals are made up from multiple domains that are slightly differently oriented. Indeed, the algorithm can comfortably deal with multiple crystals with very different orientations, unit cells and/or space groups.

The authors of the paper propose the new lattice filter as a powerful tool for processing very noisy images with crystal factors (and thus the phase information) hidden within them. The filter is able to discriminate between noise images and the very noisy images with very low contrast which contain crystal-like structures. The lattice filter retains the shape of the spots in Fourier space and also retains any phase gradients within the Bragg spots (which determine the domain structure within the crystal). Thus, it retains all of the significant information from the Bragg spots. This will open the way to combining the phases acquired from stationary, two-dimensional images with intensities of rotation diffraction data taken from the same type of crystals. In this way, the authors expect to be able to phase the diffraction information of protein and peptide crystals.