3-D human skin maps aid study of relationships between molecules, microbes and environment

"This is the first study of its kind to characterize the surface distribution of skin molecules and pair that data with microbial diversity," said senior author Pieter Dorrestein, PhD, professor of pharmacology in the UC San Diego Skaggs School of Pharmacy. "Previous studies were limited to select areas of the skin, rather than the whole body, and examined skin chemistry and microbial populations separately."

To sample human skin nearly in its entirety, Dorrestein and team swabbed 400 different body sites of two healthy adult volunteers, one male and one female, who had not bathed, shampooed or moisturized for three days. They used a technique called mass spectrometry to determine the molecular and chemical composition of the samples. They also sequenced microbial DNA in the samples to identify the bacterial species present and map their locations across the body. The team then used MATLAB software to construct 3D models that illustrated the data for each sampling spot.

Despite the three-day moratorium on personal hygiene products, the most abundant molecular features in the skin swabs still came from hygiene and beauty products, such as sunscreen. According to the researchers, this finding suggests that 3D skin maps may be able to detect both current and past behaviors and environmental exposures. The study also demonstrates that human skin is not just made up of molecules derived from human or bacterial cells. Rather, the external environment, such as plastics found in clothing, diet, hygiene and beauty products, also contribute to the skin's chemical composition. The maps now allow these factors to be taken into account and correlated with local microbial communities.

"This is a starting point for future investigations into the many factors that help us maintain, or alter, the human skin ecosystem -- things like personal hygiene and beauty practices -- and how those variations influence our health and susceptibility to disease," Dorrestein said.

Study co-authors include Amina Bouslimani, Carla Porto, Christopher M. Rath, Mingxun Wang, Yurong Guo, Nakatsuji Teruaki, Lingjuan Zhang, Andrew W. Borkowski, Michael J. Meehan, Kathleen Dorrestein, Richard L. Gallo, Nuno Bandeira, and Rob Knight, UC San Diego; Antonio Gonzalez, Donna Berg-Lyon, and Gail Ackermann, University of Colorado at Boulder; Gitte Julie Moeller Christensen, UC San Diego and Aarhus University; Theodore Alexandrov, UC San Diego, University of Bremen, Steinbeis Innovation Center SCiLS Research and SCiLS GmbH.

This research was funded, in part, by the National Institutes of Health (grants GM085764, 3-P41-GM103484 and GMS10RR029121), Howard Hughes Medical Institute, Keck Foundation, San Diego Center for Systems Biology, European Union 7th Framework Programme and Science Without Borders Program.