Plasma treatment zaps viruses before they can attack cells

The researchers prepared solutions containing adenoviruses and then treated the samples with a low-temperature plasma created by applying a voltage to a gaseous mixture in a syringe. The strong electric field energized electrons that collided with molecules in the gas, generating charged particles and highly reactive species such as oxygen atoms that likely etched away the protein shell of the viruses and damaged or destroyed the viral DNA. When the virus solutions were later added to colonies of embryonic kidney cells, the plasma-treated samples showed much less viral activity, as measured by the amount of a florescent virus protein the infected kidney cells produced. If the virus solution was covered during treatment to maximize plasma-virus interactions, more than 99 percent of the viruses could be deactivated in eight minutes.

The technique is described in a paper accepted for publication in the AIP's journal Applied Physics Letters.

Adenoviruses pose life-threatening risks to patients undergoing stem-cell therapy, so the anti-viral plasma treatment may help pave the way to safer therapies, the researchers write. Because plasma jets have multiple biomedical applications, the team is also developing a portable device that generates plasma by using a 12 V battery to decompose and ionize air, says Dr. XinPei Lu at the HuaZhong University of Science and Technology in China and leader of the team. The device might be used in rural areas and battlefields, according to Lu.