Mutation over 100 million years ago led flowers to make male and female parts differently

The findings -- published in the Proceedings of the National Academy of Sciences (PNAS) Online Early Edition -- provide a perfect example of how diversity stems from such genetic 'mistakes'. The research also opens the door to further investigation into how plants make flowers -- the origins of the seeds and fruits that we eat.

In a number of plants, the gene involved in making male and female organs has duplicated to create two, very similar, copies. In rockcress (Arabidopsis), one copy still makes male and female parts, but the other copy has taken on a completely new role: it makes seed pods shatter open. In snapdragons (Antirrhinum), both genes are still linked to sex organs, but one copy makes mainly female parts, while still retaining a small role in male organs -- but the other copy can only make male.

"Snapdragons are on the cusp of splitting the job of making male and female organs between these two genes, a key moment in the evolutionary process," says lead researcher Professor of Plant Development, Brendan Davies, from Leeds' Faculty of Biological Sciences. "More genes with different roles gives an organism added complexity and opens the door to diversification and the creation of new species."

By tracing back through the evolutionary 'tree' for flowering plants, the researchers calculate the gene duplication took place around 120 million years ago. But the mutation which separates how snapdragons and rock cress use this extra gene happened around 20 million years later.

The researchers have discovered that the different behaviour of the gene in each plant is linked to one amino acid. Although the genes look very similar, the proteins they encode don't always have this amino acid. When it is present, the activity of the protein is limited to making only male parts. When the amino acid isn't there, the protein is able to interact with a range of other proteins involved in flower production, enabling it to make both male and female parts.

"A small mutation in the gene fools the plant's machinery to insert an extra amino acid and this tiny change has created a dramatic difference in how these plants control making their reproductive organs," says Professor Davies. "This is evolution in action, although we don't know yet whether this mutation will turn out to be a dead end and go no further or whether it might lead to further complexities.

"Our research is an excellent example of how a chance imperfection sparks evolutionary change. If we lived in a perfect world, it would be a much less interesting one, with no diversity and no chance for new species to develop."

The researchers now plan to study the protein interactions which enable the production of both male and female parts as part of further investigation into the genetic basis by which plants produce flowers.

The research was supported by funding from the European Union Marie Curie Research Training Program and the Biotechnology and Biological Sciences Research Council.