Real-time Observation Of DNA-repair Mechanism

Researchers from the Kavli Institute of Nanoscience Delft are to publish an article on this in the journal Molecular Cell.

Cells have mechanisms for repairing the continuous accidental damage occurring in DNA. These damages can vary from a change to a single part of the DNA to a total break in the DNA structure. These breaks can, for instance, be caused by ultraviolet light or X-rays, but also occur during cell division, when DNA molecules split and form two new DNA molecules. If this type of break is not properly repaired it can be highly dangerous to the functioning of the cell and lead to the creation of a cancerous cell.

One major DNA-repair mechanism involved in repairing these breaks is known as homologous recombination. This mechanism has been observed for the first time by Delft University of Technology researchers in real time and at the level of a single DNA molecule.

To observe this, a DNA molecule is stretched between a magnetic bead and a glass surface. A force is exerted on the magnetic bead using a magnetic field, enabling researchers to pull and rotate a single DNA molecule in a controlled fashion. As the position of the bead changes when the DNA molecule is repaired, researchers are able to observe the repair process in detail.