Pistachios Lower Cholesterol, Provide Antioxidants

"Pistachio amounts of 1.5 ounces and 3 ounces -- one to two handfuls -- reduced risk for cardiovascular disease by significantly reducing LDL cholesterol levels and the higher dose significantly reduced lipoprotein ratios," says Sarah K. Gebauer, graduate student in integrative biosciences, Penn State, to attendees at the Experimental Biology meeting April 30 in Washington, D.C.

The researchers conducted a randomized, crossover design, controlled feeding experiment to test the effects of pistachios added to a heart healthy moderate-fat diet on cardiovascular disease risk factors. Controlled feeding experiments provide all the food eaten by study subjects for the duration of the study segment.

Participants began the study by eating an Average American Diet consisting of 35 percent total fat and 11 percent saturated fat for two weeks. They then tested three diets for four weeks each with a two-week break between each diet.

All three diets were variations on the Step I Diet, a cholesterol-lowering diet in general use. The diets included a Step I Diet without pistachios which had 25 percent total fat and 8 percent saturated fat; a Step I Diet including 1.5 ounces of pistachios per day which had 30 percent total fat and 8 percent saturated fat, and a Step I Diet including three ounces of pistachios per day which had 34 percent total fat and 8 percent saturated fat. The researchers added pistachios into the diets by including about half the amount of pistachios as a snack and by incorporating the rest into such foods as pistachio muffins, granola and pistachio pesto.

"We had really good compliance and participants were generally pleased with the diets," says Gebauer.

Standard blood tests determined the various cholesterol levels in the participant's blood after each diet. Researchers found that 3 ounces of pistachios reduced the amounts of total cholesterol in the blood by 8.4 percent and low-density lipoprotein (LDL), the so-called bad cholesterol, by 11.6 percent. The study also found that non-high density lipoproteins (non-HDL) decreased by 11.2 percent. Non-HDL levels are considered reliable predictors of cardiovascular disease risk.

The three-ounce pistachio diet also decreased the ratios of total cholesterol to HDL, LDL to HDL and non-HDL to HDL and apolipoprotein B, which are all measures of cardiovascular disease risk. "We were pleased to see a difference between the two doses of pistachios for the lipoprotein ratios because it would appear that pistachios are causing the effect and that they act in a dose dependent way," says Gebauer.

In addition, during this study researchers, researchers looked at the effects of these diets on oxidized LDL and on antioxidants in the blood.

"We were trying to see if the increased levels of antioxidants provided by pistachios could reduce inflammation and oxidation," says Gebauer. Pistachios contain more lutein, -- normally found in dark leafy vegetables -- beta carotene -- a precursor to vitamin A -- and gamma tocopherol -- the major form of vitamin E -- than other nuts. It is oxidized LDL and other lipproteins that contribute to plaque formation in arteries.

The researchers reported that both the 1.5 and 3 ounce pistachio diets reduced oxidized LDL compared with the baseline diet. Pistachio-enriched diets also resulted in significantly higher levels of lutein in the blood. The increased lutein from the 3-ounce pistachio diet correlated with a reduction in oxidized LDL which may indicate that the lutein in pistachio nuts improves the risk of cardiovascular disease by reducing serum oxidized LDL.

"Our study has shown that pistachios, eaten with a heart healthy diet, may decrease a person's CVD risk profile," says Dr. Penny Kris-Etherton, distinguished professor of nutrition and primary investigator of the study.

Researchers conducting the cholesterol study included Gebauer; Kris-Etherton; Colin D. Kay, former Penn State post-doctoral associate now at the University of East Anglia; UK; Sheila G. West, associate professor of biobehavioral health, all at Penn State, and P. Alaupovic, Oklahoma Medical Research Foundation, Oklahoma City. Researchers conducting the oxidation study included Kay, Gebauer, West and Kris-Etherton. The California Pistachio Commission supported this study. Partial support also came from the NIH-supported GCRC at Penn State.