Scientists Discover Gene "Signature" For Tumor's Tendency To Spread

Most cancer deaths are caused not by the original or primary tumor but by the metastasizing of tumor cells to other organs. Until now, cancer specialists have viewed the development of metastasis as an essentially random and unpredictable event.

But that notion is thrown into question with the new finding of a genetic "signature" – a certain pattern of activity in a handful of genes – in some solid tumors that appears to preordain them to spreading dangerously. This signature is present in the early stages of the cancer, well before there is any evidence of metastasis, say the researchers.

"These results strongly support the idea that some primary tumors are pre-configured to metastasize, and that this propensity is detectable at the time of initial diagnosis," says Sridhar Ramaswamy, MD, a researcher at Dana-Farber.

Though metastasis is a common and feared event in cancer patients, what triggers it is poorly understood. Cancer cells must undergo a series of changes to become metastatic: they have to acquire the ability to grow while unattached to any tissue, they need to bore through vessel walls to reach the bloodstream or lymphatic system, and they must develop a new blood supply in order to grow. These events are under the control of different genes.

According to the traditional view, a tumor becomes metastatic more or less by chance as a result of a very few maverick cells developing the ability to spread. "It was thought that there was a randomness to it, as new mutations would be constantly arising and some would confer metastatic potential on the tumor," says Ramaswamy.

By contrast, the researchers say their discovery reveals that the tumor is "encoded" with metastatic potential from early on by a certain group of genes, and that this same group is found in a variety of tumor types.

Ramaswamy and his colleagues compared genetic patterns in samples of primary tumors and samples of metastatic cancers. They were seeking genetic differences that might explain why primary cancers stay put while metastatic clumps of cells break off and migrate through the body.

The scientists analyzed the genetic profiles of 64 primary tumors (adenocarcinomas) and 12 samples of cancers that metastasized from other adenocarcinomas. This analysis revealed a set of 128 genes whose expression pattern differed between the primary tumors and the metastases. The scientists were puzzled by instances in which the 128-gene signature associated with the metastatic cancers was also found in some primary tumors. They concluded, though, that the gene expression program of metastasis may already be present in the bulk of some primary tumors at the time of diagnosis.

Further support for this idea came from analysis of samples from 62 lung cancers, which found the metastasis gene signature in many of them. Next, they hypothesized that lung cancer patients whose primary tumors contained the metastatic signature would do more poorly (would survive a shorter time) than those whose tumors lacked the signature. They found that this was the case.

Finally, the scientists looked in the data for a smaller set of genes that could distinguish between primary and metastatic cancer samples. They found a group of 17 genes whose activity pattern could make the distinction, and they tested it in breast cancers, prostate ca