Survey of salmonella species in Staten Island Zoo's snakes

To better understand the variety of Salmonella species harbored by captive reptiles, Staten Island Zoo has teamed up with the microbiology department at Wagner College. Eden Stark, a graduate student on the project, her advisor, Christopher Corbo, and the zoo's curator and head veterinarian Marc Valitutto want to know how many Salmonella species live among the Staten Island Zoo rattlesnakes. The zoo has a long history of exhibiting one of the most comprehensive rattlesnake collections in the world, currently with 21 of 38 species on display.

So far, Stark has surveyed 26 species of snakes. "Few other institutions have undertaken such broad scale analysis of Salmonella in snakes," notes Valitutto. The research will be presented at the American Society for Biochemistry and Molecular Biology (ASBMB) Annual Meeting during Experimental Biology 2015.

In particular, the investigators are on the lookout for pathogenic species of Salmonella, such as Salmonella arizonae. This species of Salmonella has been known to cause infections in snakes called osteomyelitis. It has a predilection for the bones, such as the vertebra. The bone may deform, and as the infection spreads, the deformed vertebrae may stop the snake from slithering.

The infection may be surgically removed or treated with antibiotics if it's localized and caught early enough. But if left untreated, the infection may eventually cause the snake to die.

"If we do get a snake that is positive for arizonae, we're concerned," says Valitutto. "We would not want add something like that to our collection because there's a possibility it will infect our other reptiles."

Another reason to account for the different Salmonella species is for the safety for the zookeepers. Salmonella "is strictly a pathogen for humans. It's something that anyone who handles reptiles, even people who keep them at home as pets, has to be very cautious about in handling them or anything that is part of their enclosure," says Corbo.

To categorize the Salmonella species, Stark isolated the bacteria from snake fecal samples. The feces were collected by seasoned zookeepers at Staten Island Zoo who know how to handle venomous snakes.

As expected, because snakes are natural hosts for Salmonella, Stark found a large number of Salmonella species in the fecal samples. She did find several species of Salmonella that are well-known as human pathogens, such as Salmonella typhimurium which can cause diarrhea, abdominal cramps, vomiting and nausea for about a week.

In the few cases where Stark possibly detected the snake pathogen S. arizoniae, the news was interesting to the zoo because the snakes weren't showing any symptoms evidence of disease. "It's important for keepers to know that a particular species of snake is carrying a potential pathogen so they can keep an eye on it," says Corbo.

Corbo adds that the handlers will now know that the tools they use to handle the snakes harboring S. arizonae need to be cleaned with extra care so that they don't accidentally infect other reptiles, especially snakes.

Stark is now delving further into the analysis with the polymerase chain reaction. She is testing each Salmonella species she isolates with the technique to see if the bacteria are expressing proteins known as virulence factors. This detail is important because not every potential pathogen will express virulence factors. The bacteria only become a problem if and when they turn on the expression of virulence factors and become infectious agents (for this reason, Salmonella arizonaie within snakes can even be further subdivided into more pathogenic serotypes).

"You can have a species and a subspecies of Salmonella that may not carry the specific genes to cause an infection," says Corbo. "Just because the species is there, it doesn't mean it's a pathogenic form of that species. That's why it takes a lot of screening to figure out exactly what we are seeing: Is it a true pathogen with the genes necessary to be pathogenic?"

Valitutto says the information from the analysis is critical for the zoo's biosecurity protocol. As he notes, "We don't want to send animals to other institutions that may be harboring a pathogenic strain of Salmonella."