Fertilizer and fuel: Nitrogen-fixing enzyme also produces hydrocarbons

They possess the enzyme nitrogenase, which combines nitrogen with hydrogen to form ammonium. In a new study, Prof. Dr. Oliver Einsle and Dr. Thomas Spatzal have not just contributed to the further elucidation of how this enzyme functions, but also described a unique mechanism it uses to produce hydrocarbons.

"We want to understand the reactions of nitrogenase in order to make the enzyme available for future biotechnological applications. At present, half of humankind can only be fed through the use of fertilizers in agriculture. This consumes roughly one percent of the world's energy production," explains Einsle. The researchers demonstrated for the first time how nitrogenase converts carbon monoxide. This leads to the production of molecules that resemble biofuels. "Thus, the enzyme is also interesting for sustainable energy production," says Einsle.

Einsle is studying the microstructure of the heart of the enzyme: a large metal core called the iron molybdenum cofactor (FeMoco). Einsle, Spatzal, and Prof. Dr. Douglas Rees in Pasadena, USA, have obtained a crystal structure that shows how a carbon monoxide molecule (CO) binds to FeMoco. "There, it unexpectedly pushes away a sulfur atom that had previously occupied the same position in the metal core. For the first time, this allows us to draw inferences on how the core reacts with other molecules," describes Einsle. The researchers published their findings in the journal Science.

"A chemical rearrangement of this kind has never been observed before in a biological system," explains Einsle. It has been known since 2010 that CO, an inhibitor of Nitrogenase, is slowly converted to hydrocarbons to a minimal extent. By applying CO gas to the enzyme during the nitrogenase reaction, the researchers found a binding site for CO and succeeded in documenting the rearrangement. Thus, in addition to the so-called "Haber-Bosch process of nitrogen fixation," nitrogenase also stimulates a reaction corresponding to the "Fischer-Tropsch synthesis of hydrocarbons," which can be used on a large scale to synthesize fuels, for instance from industrial waste gases.. "The new structural analysis is the first ever description of the mechanism of this unusual reactivity," says Einsle.

Oliver Einsle serves as professor at the Institute of Biochemistry of the University of Freiburg and is a member of the Cluster of Excellence BIOSS Centre for Biological Signalling Studies of the University of Freiburg. Thomas Spatzal completed his dissertation in Freiburg and is now conducting research with Douglas Rees at the California Institute of Technology in Pasadena, USA.