Cell's Powerhouses Dismantled: Complete Inventory Of All Proteins In Mitochondria

Their research findings are being published in the journal Cell.

During their research, the scientists encountered the Icp55 enzyme, which fulfills an important role in the stabilization of the proteins in the mitochondria. Until now, it was unclear just how the cell accomplished this. Icp55 turns out to be a molecular pair of scissors that snips off one of the ends of certain proteins, so that these proteins are transformed from an unstable to a stable form.

"This is a crucial step for fundamental research on proteins, the building blocks of life," says Kris Gevaert, VIB researcher at Ghent University. "It's the first time that such a precise protein determination of the mitochondria has been achieved. Our breakthrough was made possible by COFRADIC, a technology that enables us to analyze protein mixtures much more sensitively and accurately."

"We're now able to detect protein forms that were simply overlooked before," Kris Gevaert continues. "So we're receiving requests for scientific collaborations from all over the world."

COFRADIC is a technology developed by VIB/Ghent University researchers Joël Vandekerckhove and Kris Gevaert. With COFRADIC, the traditional conception of protein identification has been turned upside-down. Instead of first separating the intact proteins, the entire protein mixture is cut into small pieces (peptides) − which are more soluble and much easier to separate in order to conduct further analyses. Applying this approach to the samples provided by the German scientists revealed a new protein (Icp55) − which proved to be key in explaining protein stabilization in mitochondria, a mystery that has been unsolved up to now.